Detection and Inactivation of Pathogens of Epinephelus Roes at Low Concentration by a Remote and Automatic System

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基本資料

Project title

Code/計畫編號

NSC102-2221-E019-027

Translated Name/計畫中文名

低濃度石斑魚卵病原菌遠端自動化檢測暨滅菌系統(I)

Project Coordinator/計畫主持人

Chih-Wei Wu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=3088685

Year

2013

Start date/計畫起

01-08-2013

Expected Completion/計畫迄

01-07-2014

Bugetid/研究經費

713千元

ResearchField/研究領域

漁業
電子電機工程

"本研究擬以三年的時間，完成『低濃度石斑魚卵病原菌遠端自動化檢測暨滅菌系統』研究計畫。傳統之病原菌檢測方式，皆由人員至現場檢測或採樣後攜回實驗室進行病原菌分離培養，所需之時間，樣本採集、保存、搬運所造成的污染，都將影響檢測結果之準確性，且需昂貴設備方能為之。若能於養殖環境現場進行檢測，且在發現病原菌後自動進行滅菌處理，將可提高石斑魚卵之孵化率；若無發現病原菌，實無投餵藥劑之必要，如此可減少抗生素殘留的問題，以維護國人衛生安全。本計劃目標在於將實驗室晶片技術應用於石斑魚卵病原菌檢測與滅菌處理，檢測系統包含微閥門、分離純化系統、斜結構微混合器、LED 光源、液態波導管、光學偵測系統等；滅菌系統包含三維水膠滅菌複合材料、LED 可見光源、幫浦等。系統將主動吸取微量待測樣本並注入檢測晶片內，經由分離純化系統將樣本內之病原菌順利分離純化；隨後開啟微閥門並利用微混合器使病原菌與事先標定螢光分子之抗體充分反應後，進入光學檢測區內；以 LED 激發螢光分子且由液態波導管將有限之螢光訊號完整地傳送到檢測端，藉由量測螢光強度即可達到低濃度病原菌即時檢測之目的。若病原菌之濃度高於設定值，系統則主動將全部樣本注入滅菌系統內，藉由含奈米銀與二氧化鈦顆粒之三維水膠滅菌複合材料，配合適當波長之可見光光源照射以進行滅菌處理，並再次由檢測系統確認滅菌結果，直到完全淨化為止。因本研究使用水膠材料製作微閥門、分離純化系統、斜結構微混合器、滅菌複合材料等重要元件，故計畫第一年之工作重點為充分掌握水膠材料特性(組成成分比例最佳化)與機械性質(降伏強度、膨脹收縮率、疲勞測試)，進而建立水膠形變數學模式以供計畫第二年製作水膠微結構之參考。計畫第二年將以水膠製作微閥門、分離純化系統、斜結構微混合器、滅菌複合材料等重要元件並評估個別性能，亦需完成液態波導管製作；第三年將所有元件整合於檢測與滅菌系統內，另結合無線模組與可程式化自動控制器以完成病原菌遠端自動化檢測暨滅菌系統，並實際進行低濃度石斑魚卵病原菌檢測與滅菌，更將與傳統技術進行交叉比對，進而驗證系統之整體性能與準確性。就學術研究而言，本研究所提出之『分離純化系統』、『斜結構微混合器』、『三維水膠複合滅菌材料』、『低濃度石斑魚卵病原菌遠端自動化檢測暨滅菌系統』皆極具學術發表與專利申請之價值。此外，所需之反應劑量極少，故可降低檢測成本，對學術研究與產業之貢獻將更為可貴。而藉由與本校水產養殖學系周信佑教授合作，亦可有效提升跨領域的學術研發能力。就水產養殖產業而言，依據農委會九十九年的統計資料，台灣石斑魚養殖的魚塭總面積約為 2338 公頃，略估一年的產值約為新台幣 36 億台幣，此系統每提高一成的孵化率與存活率，即可提高約 3.6 億台幣產值。而即時進行病原菌檢測，另可降低藥物或抗生素使用，以維護消費者食用水產品之安全性。故本計畫除了具有學術研究價值外，對整體養殖產業亦有極高之助益，更可開創一個全新之水產病原菌檢測暨滅菌系統產業，相信不論是學術研究或產業應用都具有極高的價值。" "In this three-year project, a remote, portable, and automatic system will be designed and fabricated by MEMS technologies and be using to detect and inactive pathogens of Epinephelus roes at low concentration. The conventional techniques capable of analysis of aquatic pathogens are laboratory analysis after disease outbreak. It poses severe challenges in sampling cost, storage, transportation, and contamination. Meanwhile, aquatic pathogens can not be inactivated immediately in accordance with the detection results. The main purpose of this project is to develop a remote and automatic system for pathogens inspection and to inactive them instantly if they are found. The detection and inactivation system consists of detection system, inactivation system, micropumps, battery, wireless module, NI CompactRIO, and human-machine interface. The detection system is composed of light-emitting diode (LED), photodiode, and a microfluidic chip including microvalves, separation and purification device, active-passive micromixer, liquid core wave guide. The inactivation system is composed of LED and another microfluidic chip with hydrogel-TiO2-Ag composite materials. The system will automatically take sample by a micropump, and pathogens will be separated and purified by hydrogels with nano poles. Purified pathogens will flow into the individual mixing area and mix completely with antibody coated with fluorescence molecules via microvalve and active-passive micromixer. Eventually, we can figure out pathogen concentrations by means of the light intensity of fluorescence and NI CompactRIO. If pathogen concentration is larger than permissible value the inactivation LED will be turn on and another pump will transport Epinephelus roes into inactivation chip to kill pathogens. The detection system will be used again to confirm that pathogen concentration is less than permissible value. Finally, detection and inactivation results will be sent to user’s computer or cell phone by a wireless module. In the first year we need to figure out the characteristics (constituent optimization) and mechanical properties (yield stress, swell ratio, and response time) of different hydrogels because we use them to manufacture major devices, including microvalves, separation and purification structures, active-passive micromixer, and inactivation composite material. Furthermore, the simulation model will be constructed using the characteristics and mechanical properties developed in first year to ensure the major devices will be successfully fabricated in the second year. Each component like microvalve, separation and purification structures, and active-passive micromixer will be fabricated, tested, and integrated in the detection and inactivation chips respectively in the second year. In the third year the detection and inactivation chips, optical detection system, LED, pumps, and NI CompactRIO will be used to construct the remote, portable, and automatic system to detect and inactive pathogens of Epinephelus roes at low concentration. Finally, the results will be compared to conventional techniques to demonstrate that the simple, accurate and cheap inspection is possible by the system. As regards academic research, 『separation and purification system』,『3D active-passive micromixer』, and 『3D hydrogel composite inactivation material』 are all high novelty to apply for patents and papers. In addition, necessary expensive reagent dosage reduces, that will decrease the traditional measurement cost. Meanwhile, according to the statistical data which the Agricultural Commission announced in 2010, this output value of Epinephelus of Taiwan is slightly estimated as 3.5 billion Taiwan dollars. Hatchability will be remarkably increase if pathogens of Epinephelus roes could be inactivated completely to increase output value of Epinephelus. In summary, the main purpose of this project is trying to develop the brand-new detection and inactivation system for pathogens of Epinephelus roe to overcome the choke point of the past, and offers the method to reduce the measurement cost. Furthermore, the proposed new concept is expected to be extremely useful in academic research and the industry application."

Keyword(s)

病原菌
石斑魚卵
分離純化系統
主被動微混合器
水膠複合材料
pathogen
Epinephelus roe
separation and purification system
active-passive micromixer
hydrogel composite layer

DSpace CRIS

Detection and Inactivation of Pathogens of Epinephelus Roes at Low Concentration by a Remote and Automatic System

基本資料

Description