Wireless Biosensor System Integrated with a Microinjection System for Real-Time Monitoring of Blood Glucose/Cholesterol Levels and Drug Microinjection in Grouper Fish

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簡歷

基本資料

Project title

Code/計畫編號

MOST104-2221-E019-011

Translated Name/計畫中文名

裝戴於石斑魚魚體之整合遠端無線藥物注射與生理狀態無線感測裝置之開發

Project Coordinator/計畫主持人

Shih-Hao Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=11580742

Year

2015

Start date/計畫起

01-08-2015

Expected Completion/計畫迄

01-07-2016

Bugetid/研究經費

725千元

ResearchField/研究領域

醫學工程
藥學

"石斑魚是台灣重要內外銷經濟魚種，近年來常受到魚類病原菌感染和寒害的影響，魚類病原菌感染的解決方法主要靠抗生素為手段，養殖業者會在平時即注射抗生素做預防。而採用注射抗生素方式常無法有效控制注射劑量、易造成抗生素殘留，缺乏可即時回饋魚體生理狀態的感測裝置，作為養殖業者在正確時間內施打染病魚體適當的藥物劑量。考量魚體內血液中膽固醇與血糖的顯著驟降變化是生理狀態異常與抗病原菌感染能力降低的一個有用的指標，本研究擬以二年期進行裝戴於石斑魚魚體之整合遠端無線藥物注射與生理狀態無線感測裝置之開發並實際應用於石斑魚養殖場進行田野性能測試。預計開發出探針型酵素電極感測器並連接到無線訊號傳輸裝置，藉以即時感測魚體內血糖/膽固醇的變化，此探針型酵素電極感測器將以人為方式由外部植入魚眼球後方鞏膜內，可接觸眼球鞏膜組織間液並進行血糖/膽固醇的量測，眼球鞏膜組織間液為透明組織液，不會造成血液凝固或球蛋白凝結於感測器探針上干擾電流訊號。藉由探針型酵素電極感測器進行定期生理檢測，一旦偵測到異常狀態，可即時啟動遠端無線藥物注射裝置，經由植入魚眼球後方鞏膜內的注射針進行緊急注射抗生素或預防性藥物等，能有效控制注射劑量減少抗生素殘留，且遠端無線藥物注射裝置可進行多次注射，無須傳統人工方式多次捕撈、麻醉後注射方式，易造成魚隻受緊迫驚嚇而死亡。第二期為能提供遠端無線控制裝置所需電力，同時也將開發探針式葡萄糖氧化酵素燃料電池，此探針將由外部植入魚眼接觸眼球鞏膜組織間液，催化葡萄糖產生電力，作為裝置的輔助電力來源，提供一個可能解決電池快速消耗的問題，第二期也將著重在實際應用於石斑魚養殖場進行性能測試。" "Periodic checks of fish health and the rapid detection of abnormalities are thus necessary at fish farms. Blood glucose or plasma total cholesterol levels are significantly related to fish mortality following bacterial challenge, and are thus a good indicator of the general health of fish. In this project, we plan to develop a wireless enzyme sensor system integrated with a wireless microinjection system to determine blood glucose or cholesterol levels in fish (Taiwan Grouper) and inject antibiotic when extraordinary condition is detected. The wireless enzyme biosensor was constructed with Pt-Ir wire as the working electrode and Ag/AgCl paste as the reference electrode. To avoid blood coagulation and proteins coalescing on the sensor, we plan to implant the sensor in the fluid under the scleral surface of the eyeball (EISF). The EISF is presumed to reflect the levels of most blood components and does not include the substances contained in blood that inhibit sensor measurement. When extraordinary condition of glucose or cholesterol levels is detected, we can immediately activate the wireless microinjection system to inject a proper dose of antibiotic into the eyeball sclera by an implanted microneedle. Our proposed method can effectively reduce the antibiotic residue in a fish body. To provide the electrical power for wireless enzyme sensor, we plan to develop and integrate an enzymatic biofuel cell into the wireless enzyme sensor. The needle anode is inserted into fish eye ball to contact with the EISF to produce electrical power. We believe that our proposed wireless system can provide a useful tool to rapidly check fish health at fish farms."

Keyword(s)

石斑魚
眼球鞏膜組織間液
酵素電極感測器
遠端無線藥物注射
Taiwan Grouper
eyeball interstitial sclera fluid
wireless enzyme biosensor
wireless microinjection system

DSpace CRIS

Wireless Biosensor System Integrated with a Microinjection System for Real-Time Monitoring of Blood Glucose/Cholesterol Levels and Drug Microinjection in Grouper Fish

基本資料

Description