Sex Differentiation Measurement of a Living Fish: Wireless Biosensor System for Real-Time Monitoring of Blood Steroid Hormones Levels in Grouper Fish

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Project title

Code/計畫編號

MOST107-2221-E019-027

Translated Name/計畫中文名

活體量測魚類性別轉變：開發可自體產生電力之植入式魚類性成熟脂醇荷爾蒙無線感測裝置

Project Coordinator/計畫主持人

Shih-Hao Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=12677646

Year

2018

Start date/計畫起

01-08-2018

Expected Completion/計畫迄

01-07-2019

Bugetid/研究經費

771千元

ResearchField/研究領域

機械工程

某些魚類在一生中，會經歷雄魚與雌魚二種角色，若能了解個中轉變的因素，就能左右其性別，進而在產業經濟上發揮貢獻，如：石斑魚是台灣重要內外銷經濟魚種，石斑魚原先發育是雌魚，約五至九年間才能性轉變為雄魚，導致大量用於人工繁殖的雄魚必須靠人工促進性轉變而來。魚類生殖內分泌系統控制著魚類性成熟與性別轉變，可刺激生殖腺合成3 種性類脂醇荷爾蒙(steroid hormone)，包括：雌性醇(Estrogen)、睪固酮 (Testosterone)、排卵素 (DHP)，而雌性醇與睪固酮可促進卵巢和精巢的形成。因此，開發一個方法，可隨時監控魚體內睪固酮(T)與雌性醇(E)的作用和動態變化，為正確掌握魚類性轉變現象的發生不可或缺的工作，目前的研究資料指出：血漿內睪固酮(T)濃度或睪固酮(T)與雌性醇(E)的比值(T/E)和石斑魚性轉變成雄魚有高關聯性。第一年：本研究擬進行開發裝戴於石斑魚魚體之可自體產生電力之植入式魚類性成熟脂醇荷爾蒙無線感測裝置，可即時活體監控魚體內睪固酮(T)與雌性醇(E)的動態變化，預計開發出兩款探針型酵素電極感測器，可同時量測魚體內睪固酮(T)與雌性醇(E)的變化，並連接到無線訊號傳輸裝置，此探針型酵素電極感測器將以人為方式由外部植入魚眼球後方鞏膜內，可接觸眼球鞏膜組織間液(eyeball interstitial sclera fluid, EISF)並進行睪固酮(T)與雌性醇(E)的量測，眼球鞏膜組織間液(EISF)為透明組織液，不會造成血液凝固或球蛋白凝結於感測器探針上干擾電流訊號而無法長期量測。此外，為能提供遠端無線控制裝置所需電力，同時也將開發探針式葡萄糖氧化酵素燃料電池，此探針將由外部植入魚眼接觸眼球鞏膜組織間液，催化葡萄糖產生電力，作為裝置的輔助電力來源，提供一個可能解決電池快速消耗的問題。第二年：擬利用第一年所開發的荷爾蒙無線感測裝置探討人為荷爾蒙用藥(芳香化酶抑制劑，Fadrozolehydrochloride hydrate)如何降低魚體內雌性醇分泌，從而提高魚體內雄激素對雌激素的比例、誘導魚體向雄性方向演變，以及探討人為荷爾蒙用藥狀況(種類、注射次數、注射間隔時間與所用劑量等)如何有效控制著魚類性成熟、促進性別轉變，進而在實際養殖漁業上發揮貢獻。 The rapid detection and periodic checks of fish sex differentiation play an important role at fishery economics. Blood steroid hormones such as estrogen and testosterone are significantly related to fish sex differentiation and are thus a good indicator to monitor its process of fish sex differentiation. In this two-year project, we plan to develop a wireless enzyme sensor system to determine estrogen and testosterone levels in fish (Taiwan Grouper). In first year, we will construct the wireless enzyme biosensor with Pt-Ir wire as the working electrode and Ag/AgCl paste as the reference electrode. To avoid blood coagulation and proteins coalescing on the sensor, we plan to implant the sensor in the fluid under the scleral surface of the eyeball (EISF). The EISF is presumed to reflect the levels of most blood components and does not include the substances contained in blood that inhibit sensor measurement. When the significant variation of estrogen and testosterone levels is detected, we can monitor the process of fish sex differentiation in real time. To provide the electrical power for wireless enzyme sensor, we plan to develop and integrate an enzymatic biofuel cell into the wireless enzyme sensor. The needle anode is inserted into fish eye ball to contact with the EISF to produce electrical power. In second year, we will investigate the effect of artificial hormone (Fadrozolehydrochloride hydrate) on the process of fish sex differentiation in real time by using a wireless enzyme sensor system we develop in the first year. We believe that our proposed wireless system can provide a useful tool to detect fish sex differentiation process in aquaculture industry.

Keyword(s)

石斑魚
眼球鞏膜組織間液
酵素電極感測器
雌性醇
睪固酮
Taiwan Grouper
eyeball interstitial sclera fluid
wireless enzyme biosensor
estrogen
testosterone

DSpace CRIS

Sex Differentiation Measurement of a Living Fish: Wireless Biosensor System for Real-Time Monitoring of Blood Steroid Hormones Levels in Grouper Fish

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