Preparation Photothermal Therapy of Multifuctional Mesoporous Silica Nanoparticle as Theranostic Drug Delivery System

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Project title

Code/計畫編號

MOST103-2113-M019-001

Translated Name/計畫中文名

製備光熱治療診斷之多功能二氧化矽奈米粒子

Project Coordinator/計畫主持人

Hsiu-Mei Lin

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=8312184

Year

2014

Start date/計畫起

01-08-2014

Expected Completion/計畫迄

31-07-2015

Bugetid/研究經費

1200千元

ResearchField/研究領域

化學

"奈米粒子顯影劑因在生醫成像及治療診斷學上的多種應用而備受重視。近年來，科學家們對於以多功能二氧化矽奈米粒子(mesoporous silica nanoparticles, MSN)在成像及治療上的雙效應用展現出明顯的研究興趣。這個領域迅速擴展，產生出一個新的術語：「治療診斷學」(syn: theranostic)，這個術語是用來形容作為診斷和治療劑的雙重角色平台。與傳統慣用的顯影劑相比，二氧化矽奈米粒子擁有多種潛在優勢，此奈米粒子能夠經由設計合成出多功能二氧化矽奈米粒子，改善細胞內檢測的靈敏度且能增強標靶的效率；此外，為了增強藥物載體的治療能力，本次的研究將以金奈米粒子(AuNP) 作為核心，藉由金奈米粒子可於近紅外光區吸收紅外光並轉化成熱能的特性，以「光熱治療」法同時與化學療法結合，之後再披附上二氧化矽的外殼，製備出以金奈米粒子為核心之複合多功能二氧化矽奈米粒子 (gold nanoparticles@mesoporous silica nanoparticles, AuN@m-SiO2)。接著，我們將合成出以雙重顯像、細胞內控制及葉酸修飾同時具有雙重治療與診斷功能的多功能金奈米粒子複合中孔二氧化矽奈米粒子，希望此多功能的奈米藥物載體能應用在生醫成像及治療診斷學，這種組合的多功能奈米粒子能夠同時在患部累積足夠的劑量當作顯影劑及治療藥劑，使它們在治療診斷學上大有可為。在此研究中，我們將進行四個部分的研究。首先，合成出AuNP，並以MSN包覆AuNP 後形成核-殼結構。接著，MSN外殼會同時參雜Eu3+與Gd3+進入MSN孔壁中，合成出 AuN@m-SiO2:EuGd，使MSN同時具有螢光與磁性的雙重顯影功能；第三部分則以 AuN@m-SiO2:EuGd奈米粒子為基礎，將細胞內控制釋放藥物嫁接上奈米粒子的孔壁上；最後將於AuN@m-SiO2:EuGd/藥物奈米粒子表面修飾上葉酸分子，根據之前的研究經驗以最佳化條件來結合雙重顯影、細胞內控制與葉酸三者到中孔洞奈米藥物載體上，置備出多功能金奈米粒子複合中孔二氧化矽奈米粒子(AuN@m-SiO2:EuGd/drug/folate) 並探討其物性、化性，進行in vitro和in vivo的測試。" "The use of nanoparticles imaging agents in theranostic and biomedical imaging is receiving considerable attention in recent years, the research teams involving physicists and material chemists are interest in the multifunctional mesoporous silica nanoparticles (MSNs) because of the dual-model for imaging and therapy. This field is expanding rapidly, bringing a new term: theranostic, which means a combination of therapeutic and diagnostic. Comparing with the traditional imaging agent, the MSNs have many advanced features to develop into a multifunctional platform by elaborate design, in order to improve the sensitivity of cellular examination and the effect of targeting. Furthermore, in order to enhance the ability of cancer treatment, our drug carrier is going to use gold nanoparticles (AuNPs) as the core in this study. Due to the gold nanoparticle can absorb infrared light in the near infrared region and convert it into heat, photothermal therapy have been published for cancer therapy. Then, we are going to coat silica shell on the surface of gold nanoparticles to prepare the complex multifunctional silica nanoparticles with gold nanoparticles core (AuN@m-SiO2). The multifunctional drug nanocarrier will combine dual imaging, cellular control and folate-modified, and containing the ability of therapy and diagnosis to apply to theranostic and biomedical imaging. This combinative multifunctional drug nanocarriers being imaging and therapeutic agent can accumulate a sufficient amount at lesion, with great prospects of theranostic in the future. This research conducts with four parts: first, AuNPs are going to be synthesized. Then, we doped Eu 3+ and Gd 3+ into the MSNs to synthesize the EuGd-MSN with fluorescence and magnesium dual imaging function. This silica material will coat on the surface of AuNP to be AuN@m-SiO2:EuGd; in the third section, we will conjugate the intracellular-controlled drug on AuN@m-SiO2:EuGd; finally, the folate molecular is going to graft on the surface of AuN@m-SiO2:EuGd/drug. According to our research experience, we can bring the dual imaging, cellular control and folate together by optimal conditions to prepare the multifunctional drug nanocarrier. We will respectively investigate the physical and chemical properties of AuN@m-SiO2:EuGd/drug/folate, and test the in vitro and in vitro assessment."

Keyword(s)

葉酸標定
癌症治療
藥物控制釋放
智慧型藥物遞送系統
細胞內控制藥物
遞送系統
雙重顯影
標靶治療
診斷治療奈米粒子
光熱治療
in vitro
in vivo
Folate-targeting
Cancer Therapy
Drug Controlled Release
Intelligent Drug
Delivery
Intracellular-controled Drug Delivery System
Dual Imaging
Targeting Therapy
Photothermal therapy
Theranostic Nanoparticles

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Preparation Photothermal Therapy of Multifuctional Mesoporous Silica Nanoparticle as Theranostic Drug Delivery System

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