The Functional Roles of Single-Minded Gene and Toxicities of Environmental Hormones in Zebrafish Head Skeleton Development (II)

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Project title

Code/計畫編號

NSC92-2313-B019-031

Translated Name/計畫中文名

Single-Minded基因與環境荷爾蒙戴奧辛對斑馬魚頭骨發育的影響(II)

Project Coordinator/計畫主持人

Chin-Hwa Hu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=848933

Year

2003

Start date/計畫起

01-08-2003

Expected Completion/計畫迄

01-07-2004

Bugetid/研究經費

1242千元

ResearchField/研究領域

生物技術(理)
生物科學

本實驗室在先前的研究中發現斑馬魚的sim2基因在發育初期即在間腦，腦室，鰓弧以及腸胃等處有相當專一性的表現。在本計畫的第一期實驗中，即探討了sim2在斑馬魚頭骨發育的過程中所扮演的角色。在本期的計畫實驗中，我們主要在探討環境荷爾蒙戴奧辛對於頭骨發育的影響，發現了戴奧辛會造成斑馬魚頭骨機型發育的情況，這項研究結果稍早由Teraoka等人發表（Toxicol Sci. 65,192-199）。但是本實驗室在研究過程中發現色素抑制劑phenylthiourea (PTU)會與戴奧辛產生擷抗作用，明顯降低戴奧辛所誘發的cyp1a1基因的活性。這項實驗推翻了一般對於PTU的看法，認為PTU對於斑馬魚胚胎不會產生影響的推論。在實驗中，我們發現PTU本身會誘發較低量的cyp1a1基因的表現，而這項基因的調控是受到AHR所控制。這項研究將於今年七月發表於Biochemical Pharmacology。本計畫第三期，我們將持續對sim2以及arnt2基因對於腦神經與頭骨的發育進行持續的研究，預期將會有所突破。During embryogenesis, sim2 mRNA appears in the animal hemisphere as early as 3 hpf and is expressed in a widespread pattern throughout the epiblast at the 75% epiboly stage, During the segmentation stae, sim2 mRNA is prominently expressed in the primordium of the hindbrain and appears as a transverse stripe in the epithelial layers of the mid-diencephalic boundary (MDB). In sim2-knockdown embryo, the craniofacial skeleton development was disrupted. To reveal the mechanism of TCDD-mediated defect in fish embryo, we have investigated the head skeleton development in TCDD-treated embryo by Alcian blue stain. It appeared that the development of cranial skeleton was disturbed. In addition, we found phenylthiourea (PTU) blocked TCDD-mediated cyp1a1 transcription via AHR signaling pathway. PTU induced a mild level of cyp1a1 transcription which was eliminated in AHR knockdown embryo. These results will be published (Biochemical Pharmacology 68 (2004) 63–71).

Keyword(s)

zebrafish
sim2
TCDD
phenylthiourea
cyp1a1
AHR

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The Functional Roles of Single-Minded Gene and Toxicities of Environmental Hormones in Zebrafish Head Skeleton Development (II)

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