The Effect of Various Manostructure of Collagen Carrier on the Adhesion, Proliferation and Differentiation of Osteoblasts-Like Cell Line MG63

View Statistics Email Alert RSS Feed

Information

Details

Project title

Code/計畫編號

NSC94-2614-B019-002

Translated Name/計畫中文名

不同奈米結構之膠原蛋白載體對類骨母細胞株MG63之貼附,增生與分化機轉的研究

Project Coordinator/計畫主持人

Fu-Yin Hsu

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

蔡曉雯

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=1150335

Year

2005

Start date/計畫起

01-10-2005

Expected Completion/計畫迄

01-07-2006

Bugetid/研究經費

700千元

ResearchField/研究領域

醫學工程

膠原蛋白為細胞外間質中最主要的成分之一，在生物組織中以非水溶性的網狀型式存在，主要功能為當作細胞生長貼附之支架、固定聚醣分子穩定組織結構、以及調節組織形成和維持細胞形態等多種功能。過去細胞培養載體多為二維之基材，無法提供細胞類細胞間質之三維生長環境。此外，貼附型細胞在放大培養時常會遇到培養面積不足之問題。本計劃利用高分子電解質錯合作用原理將膠原蛋白溶液滴入硫酸軟骨素中達到凝聚，以製備三維纖維結構之膠原蛋白顆粒。由於膠原蛋白纖維之大小、型態及排列會因製程參數的改變而有所不同，而且膠原蛋白纖維之大小、型態及排列均會影響細胞生長與分化的情況。因此，我們將在此計畫中製備具有 D-periodic的膠原蛋白纖維及不具有D-periodic的膠原蛋白纖維結構之膠原蛋白纖維奈米微結構對細胞貼附、生長與分化之影響。 "The aim of the study is to develop a novel method of preparation of collagen gel beads. According to the principle of complex coacervation, collagen beads can be formed by extruding collagen solution into chondroitin sulfate A (CSA) solution. In this work, we investigate the factors that affect formation and properties of collagen beads including the collagen and chondroitin sulfate A concentrations and pH of collagen solution or CSA solution. On the other hand, Type I Collagen is also the most commonly used biomaterial in matrix construction, and is known to affect the growth and differentiation of a variety of cells. Thus, we would like to detect how the nanostructure of this protein to influence the behaviors of cells. To this end, MG-63 are cultured on the different collagen matrix in which the collagen fibrils containing D-band or not, and use of two-photo and gene-chip to examine the effects of the fibril structure on the attachment, growth and differentiation of cells."

DSpace CRIS

The Effect of Various Manostructure of Collagen Carrier on the Adhesion, Proliferation and Differentiation of Osteoblasts-Like Cell Line MG63

Details

Description