Preparation of Dioscorea-Chitosan Nanoparticles and Their Antioxidative Effect on Skin Fibroblast Cells

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Project title

Code/計畫編號

NSC93-2313-B019-023

Translated Name/計畫中文名

山藥---幾丁聚醣奈米粒之製備及其對皮膚纖維母細胞抗氧化性的影響

Project Coordinator/計畫主持人

Ke-Liang Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=990807

Year

2004

Start date/計畫起

01-08-2004

Expected Completion/計畫迄

01-07-2005

Bugetid/研究經費

914千元

ResearchField/研究領域

食品科技(農)

" 山藥是近年來國內積極進行研究發展的重點保健用藥用植物，山藥保健食品、化妝品的開發具有極大的市場潛力。幾丁聚醣是在食品、醫藥、化工等方面都有廣泛用途的天然高分子。奈米技術可以應用原子、分子以由下而上之方式製造具有奈米結構之新產品。幾丁聚醣與奈米技術之研究已廣受世界各國所重視。山藥之奈米化產品則未曾有相關報導。本計畫擬以山藥與幾丁聚醣為原料，運用乳化凝集法將其製成奈米粒，並比較山藥萃取液、水解液、奈米粒與市售之顆粒狀維他命在抗氧化力上之差異，以評估山藥奈米粒之應用性。擬使用不同溶劑萃取山藥中所含之儲藏蛋白和多酚類化合物，並以不同蛋白.進行水解，再探討山藥萃取液、水解液清除DPPH (1,1-di-phenyl-2-picrylhydrazyl ) 自由基之能力。以抗氧化力最高之山藥溶液與幾丁聚醣製成奈米粒。接著以細胞檢驗探討山藥萃取液、水解液及山藥奈米粒對於皮膚纖維母細胞存活率、細胞增生以及促進膠原蛋白合成之影響。最後將山藥奈米粒製成凝膠狀產品以檢測其安定性。本計畫之實施一方面可以建立製備奈米化妝品之方法，另外也有助於釐清山藥奈米粒對抗氧化性之影響。本計畫之研究成果除了可建立奈米技術在藥用植物上的應用基礎之外，也可提供國內生物科技及中草藥產業開發新產品之參考。 " " Dioscorea spp. (yam) is a herbal neutraceutical that has recently received a lot of government funding and research interest in Taiwan. Market trends suggest that health foods and cosmetics made of Dioscorea may have a great potential. Chitosan is a non-toxic, biocompatible, and biodegradable natural macromolecule. It has a variety of potential applications in food, pharmaceutical, and chemical industries. Nanotechnology helps utilize atoms and molecules to produce new nanostructured products by a bottom-up approach. The fundamental and applied research of chitosan and nanotechnology has attracted much attention all over the world. However, no literature report can be found on nanosized Dioscorea product. The objectives of this project are as follows: To investigate the preparation of Dioscorea-chitosan nanoparticles by a modified emulsification - droplet coalescence method, To compare the differences between the antioxidative capabilities of Dioscorea extract, hydrolysates, Dioscorea-chitosan nanoparticles, and commercial vitamin powders, and To evaluate the application of Dioscorea-chitosan nanoparticles in a cosmetic gel formulation. We plan to use different solvents to extract the Dioscorin (storage proteins) and polyphenol from Dioscorea. Then we will hydrolyze the Dioscorin with different proteinases. Subsequently, the scavenging capabilities of Dioscorea extract, hydrolysates, and Dioscorea-chitosan nanoparticles against the DPPH (1,1-di-phenyl-2-picrylhydrazyl) free radicals will be examined. The extract or hydrolysate with the highest antioxidative capability will be made into nanoparticles. Cell assay will be used to explore the effects of Dioscorea extract, hydrolysates, and nanoparticles on the cell viability, growth, and collagen formation of cultured human skin fibroblast. A cosmetic gel will be prepared to examine the gel stability with the inclusion of Dioscorea-chitosan nanoparticles in the formula. The implementation of this project could help establish the methods of preparing nanoparticles from herbal extract. It will also help us understand the influence of nanostructure on the antioxidative capability of Dioscorea. The results of this project could help to realize the application of nanotechnology in novel product forms from herbal plant extracts. They could provide an invaluable reference protocol for developing new products in the biotechnology, herbal medicine, and cosmetic industry. "

Keyword(s)

山藥
幾丁聚醣
奈米粒
纖維母細胞
抗氧化
Dioscorea
yam
chitosan
nanoparticle
fibroblast
antioxidative

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Preparation of Dioscorea-Chitosan Nanoparticles and Their Antioxidative Effect on Skin Fibroblast Cells

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