Telomerase Activity Involved to Apoptosis of Tumor Cells by Antitumor Factor in Hard Clam

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簡歷

基本資料

Project title

Code/計畫編號

NSC88-2313-B019-050

Translated Name/計畫中文名

文蛤抗腫瘤因子誘導癌細胞程式死亡與端粒脢活性相關性

Project Coordinator/計畫主持人

Zwe-Ling Kong

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=434811

Year

1999

Start date/計畫起

01-08-1998

Expected Completion/計畫迄

01-07-1999

Bugetid/研究經費

700千元

ResearchField/研究領域

食品科技(農)

文蛤熱水抽出物對腫瘤細胞的作用非常顯著且意義特殊,目前已獲得其糖蛋白部份結構分析及活性機制探討。已知此活性成份能特異性的使肝腫瘤及結腸癌等癌化細胞、手術檢體或癌細胞株致死、經分析得知:1) 腫瘤特異性應與其主成分糖蛋白部份Mannose glycosylation結構有關,而類似凝集素WGA功能,可由其NAcGlc胺糖結合性與競爭性糖基分析解釋,2) 由細胞螢光鏡檢及染色體電泳分析,確認其致死機制乃是為一種自殺過程或稱細胞程式化死亡,並與細胞內信息傳遞Caspase-PARP degradation pathway有關,3)細胞程式化死亡發生時觀察到端粒酵素活性顯著降低。 Nondialyzable fraction of hot-water extracts of hard clam, Meretrix lusoria act as an apoptotic factor to hepatoma, HepG2 and Hep3B, and especially toward hepatoblastoma, HuH-6KK or HT-29 tumor cells. Such hot water extracts showed strong cell aggluting and stimulating activity on cultured U-M, HB4C5, and SI102 cells but not on U937 or HL60 cells. We also found telomerase activity decreasing in dose dependency. In this project, cellular DNA damage and telomerase activity in the presence of hard clam extract had been studied parallely. Telomerase activity was detected by designed telomere oligonucleotides with PCR amplification and DNA probe technique (TRAP). We also found that the antitumor activity may be through the Caspase-PARP degradation pathway, as well as NAcGlucosamine moiety on target cells.

Keyword(s)

抗腫瘤因子
細胞凋亡
端粒酶
文蛤
癌細胞
Antitumor factor
Apoptosis
Telomerase
Hard clam
Cancer cell

DSpace CRIS

Telomerase Activity Involved to Apoptosis of Tumor Cells by Antitumor Factor in Hard Clam

基本資料

Description