Development and application of the sulfate polysaccharide extracts from Sarcodia montagneana as the health care agent against PM2.5-induced pneumonia and lung cancer(1/2)

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Project title

Code/計畫編號

MOST109-2622-B019-006

Translated Name/計畫中文名

海木耳硫酸多醣萃取物做為改善PM2.5誘導肺炎及肺癌保健劑之開發與應用(1/2)

Project Coordinator/計畫主持人

Chang-Jer Wu

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13609634

Year

2020

Start date/計畫起

01-11-2020

Expected Completion/計畫迄

31-10-2021

Bugetid/研究經費

1150千元

ResearchField/研究領域

食品科技(工)

近年來，隨著健康意識抬頭，空氣污染的問題逐漸受到重視，環境中PM2.5對公眾健康造成的影響已經成為全球人們關注的焦點。近期流行病學和毒理學的研究顯示PM2.5會引起心肺功能的損傷和疾病。多個研究也指出肺癌的發生率提高和PM2.5有著密不可分的關係。在台灣，癌症一直是十大死因之首，且以肺癌的致死率最高，這可能與PM2.5污染的問題有關。在現代社會，人們越來越關注健康，也越來越關注從自然來源分離出來的保健品。近年來的研究文獻指出海藻硫酸多醣具有抗病毒、抗發炎、抗腫瘤、抗血栓、提高免疫力等保健功效。本計畫主要利用細胞實驗及小鼠動物模式探討海木耳硫酸多醣萃取物對於改善PM2.5誘導肺炎及緩解PM2.5加重肺癌的效果。首先，我們用熱水萃取法製備海木耳硫酸多醣萃取物，並對其硫酸多醣進行成分分析。接著，進行海木耳多醣萃取物處理PM2.5誘導肺炎及PM2.5加重肺癌細胞實驗，以細胞存活率、肺癌細胞遷移試驗、巨噬細胞發炎及吞噬能力、細胞激素含量變化等實驗進行改善PM2.5誘導肺炎及緩解PM2.5加重肺癌效果評估，進一步分析海木耳多醣萃取物改善PM2.5誘導肺炎及緩解PM2.5加重肺癌之機制。最後，在PM2.5誘導肺炎及PM2.5加重肺癌的C57BL/6小鼠模式中，探討口服海木耳多醣萃取物改善異PM2.5誘導肺炎及緩解PM2.5加重肺癌的效果及其作用機制。經由上述的研究，我們希望能發展出有效的海木耳硫酸多醣產品，以作為改善PM2.5誘導肺炎及緩解PM2.5加重肺癌健康食品之應用。 In recent years, growing health consciousness has led people to pay more attention to air pollution. The impacts of ambient PM2.5 on public health have become great concerns worldwide. Recently, epidemiological and toxicological studies have shown that PM2.5 induce cardiopulmonary disorders and impairments. And, more and more study indicate that PM2.5 has been associated with an increase in lung cancer incidence. In Taiwan, cancer is the first among the top ten leading causes of death, and lung cancer has the highest fatality rate. In modern society, people pay more and more attention to health, and much attention has been focused on health care agent isolated from natural sources. Recently, the sulfated polysaccharide extracts of seaweed have been shown broad biological activities, including antiviral, antitumor, immunomodulatory and anticoagulant activity. This study will investigate the sulfated polysaccharide extracts of Sarcodia montagneana against PM2.5-induced pneumonia and PM2.5-aggravated lung cancer on in vitro and in vivo model. First, we prepare sulfated polysaccharide extracts from red algae (Sarcodia montagneana) by using hot-water extraction methods and analyze their components of polysaccharides. We will use cell viability assay (MTS assay) to evaluate improvement of PM2.5-induced toxicity and inflammation by the sulfated polysaccharide extracts of Sarcodia montagneana. We will utilize transwell assay, NO assay and cytokine expression (ELISA) to study the protective effects of the sulfated polysaccharide extracts of Sarcodia montagneana. In C57B/6 mice model, we will investigate the improvement effects and its mechanisms by orally treatment with the sulfated polysaccharide extracts of Sarcodia montagneana against PM2.5-induced pneumonia and PM2.5-aggravated lung cancer. Through all these efforts, we hope to develop that sulfated polysaccharide extracts of Sarcodia montagneana may be used as the health food supplement to against PM2.5-induced pneumonia and PM2.5-aggravated lung cancer.

Keyword(s)

PM2.5
保健品
海藻
硫酸多醣
海木耳
肺炎
肺癌
PM2.5
Health care agent
Seaweed
Sulfate polysaccharide
Sarcodia montagneana
Pneumonia
Lung cancer

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Development and application of the sulfate polysaccharide extracts from Sarcodia montagneana as the health care agent against PM2.5-induced pneumonia and lung cancer(1/2)

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