The Application of Microtube Array Membrane-Immobilized Lactic Acid Bacteria in the Repeated-Batch Fermentation of Red Alga Gracilaria for Lactic Acid Production

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Project title

Code/計畫編號

MOST105-2221-E019-075

Translated Name/計畫中文名

微管束陣列膜固定化乳酸菌應用於紅藻龍鬚菜重覆批次發酵生產乳酸

Project Coordinator/計畫主持人

Hong-Ting Lin

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=11896589

Year

2016

Start date/計畫起

01-08-2016

Expected Completion/計畫迄

01-07-2017

Bugetid/研究經費

814千元

ResearchField/研究領域

食品科技(工)

聚乳酸具有生物相容性、生物降解性等特性，是目前最熱門的高分子聚合物材料之一。乳酸為最主要製備聚乳酸的原料，並廣泛被應用在工業上如製藥及食品業等。據估計，到2020年，乳酸全球需求量將達到100萬公噸，而微生物發酵為目前主要乳酸的生產方法。海藻含有高量海藻多醣以及海藻纖維且不影響糧食供應、雨林及耕地，可做為良好的乳酸發酵碳源。目前我國主要養殖的海藻以龍鬚菜較具規模，是台灣唯一可以在陸地上大量養殖之大型海藻。在發酵方式中，利用固定化細胞於重複分批發酵為一種可有效的降低生產成本及提高發酵速度的方式。聚乳酸微管束陣列膜為一種新穎的細胞固定化技術，具備可大規模量產、可回收、成本低及安定性高等優點。故本計晝擬利用聚乳酸微管束陣列膜固定化乳酸菌用於重複批次發酵紅藻龍鬚菜水解液製備乳酸。第一年研究重點著重於固定化乳酸菌的製程研究、最適包覆率及探討管壁孔隙對乳酸發酵的影響，並實際以固定化乳酸菌經由批次及重複批次發酵標準商用培養基來製備乳酸。第二年期將製備紅藻龍鬚菜水解液，並探討添加營養物質如脂肪酸對乳酸發酵的影響，最後再以固定化乳酸菌以批次及重複批次發酵標準商用培養基來製備乳酸。 "Polylactic acid is one of the most popular polymer materials due to its biocompatible, biodegradable and other characteristics. Lactic acid is not only the main raw material for the preparation of polylactic acid, but widely used in industry such as pharmaceutical and food industries. It is estimated that by 2020, global demand for lactic acid will reach 1 million tonnes, and microbial fermentation is the main approach for lactic acid production. Seaweed, which contains high amount of polysaccharides and does not affect the food supply, forests and arable land, can be used as a good carbon source for lactic acid fermentation. At present, red alga Gracilaria is the only alga to be cultivated in large scale and land-based in Taiwan. Among the fermentation methods, the use of immobilized cells in repeated batch fermentation can effectively reduce production costs and improve the speed of fermentation. Poly-l-lactic acid microtube array membrane, a novel cell immobilization technology, has several advantages, such as large scale production, recoverability, low cost and stability. Therefore, this proposal intends to use poly-l-lactic acid microtube array immobilized lactic acid bacteria for repeated-batch fermentation of red alga Gracilaria for lactic acid production. The first year of research will focus on the process for immobilization of lactic acid bacteria, the optimum encapsulation rate and investigate the effect of the pore size on lactic acid fermentation. Then Poly-l-lactic acid microtube array membrane immobilized lactic acid bacteria will be applied in batch and repeated-batch fermentation of commercial MRS broth for lactic acid production. In the second year red alga Gracilaria hydrolyzate will be prepared, and other extra nutrients, such as lipids, to be added for better lactic acid fermentation will be investigated. Then Poly-l-lactic acid microtube array membrane immobilized lactic acid bacteria will be applied in batch and repeated-batch fermentation of red alga Gracilaria hydrolyzate for lactic acid production."

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The Application of Microtube Array Membrane-Immobilized Lactic Acid Bacteria in the Repeated-Batch Fermentation of Red Alga Gracilaria for Lactic Acid Production

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