Study on the Activity of Anti-eterovirus and Application as Prebiotics of Oligosaccharides from Carrageenan and Fucoidan

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基本資料

Project title

Code/計畫編號

NSC102-2313-B019-012-MY3

Translated Name/計畫中文名

紅藻膠寡醣與岩藻寡醣之抗腸病毒活性與益生性應用之探討

Project Coordinator/計畫主持人

Chorng-Liang Pan

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=3118713

Year

2013

Start date/計畫起

01-08-2013

Expected Completion/計畫迄

01-07-2014

Bugetid/研究經費

1199千元

ResearchField/研究領域

食品科技(農)

本研究目的為探討紅藻膠寡醣與海帶岩藻寡醣之益生性、和抗血小板凝血等生理活性之影響，並經 Caco-2 細胞系統測試紅藻膠寡醣與海帶岩藻寡醣於腸道吸收與運輸之變化以及於 Caco-2 細胞模式中測試抗腸病毒之活性。在促進細菌生長試驗中，以紅藻膠寡醣 COS-3K 與添加硫酸基紅藻膠寡醣 COS-DMF 為碳源培養乳酸桿菌 Lb. acidophilus 與 Lb. plantarum 之試驗中，菌數分別為 8.46 log CFU/mL 和 8.48 log CFU/mL 與 8.32 log CFU/mL 與 8.41 log CFU/mL，結果顯示生長量均較對照組為高，且提高硫酸基之寡醣更能夠增加 Lactobacilli 之利用性，而 COS3k 與 COS-DMF 對 B. bifidum、B. adolescenis 之生長影響，與空白組比較並無顯著差異。COS-3k 與 COS-DMF 對 Clostridium perfringens 的生長影響，與對照組比以 COS-DMF 較具抑制壞菌生長之能力。以 1% FOS3k 及 1% FOS-3k-DMF 做為碳源時可促進 B. bifidum、B. adolescenis、Lb. acidophilus、及 Lb. plantarum 之生長，分別菌數可提升至 8.20-9.06 log CFU/mL，但對 Clostridium perfringens 無促進生長之作用。在 Caco-2 細胞吸收及運輸之實驗中，結果顯示 COS3k 運輸液反應 270 分鐘後，下層液中之總糖濃度由 0 mg/mL 上升至 1.804 mg/mL，細胞吸收率為 18.06%，COS3k 之運輸率為 3.46%。以 TLC 分析上下層液含糖量變化，結果顯示當反應 180 min 時才漸漸有層析波段出現，Caco-2 細胞至 180 min 後對紅藻膠寡醣才較有運輸效果。當反應 270 min 後，FOS-3k 與 FOS-3k-DMF 之 Caco-2 細胞吸收率分別約為 27.37% 與 20.52%，運輸率則為 19.07% 與 16.92%。紅藻膠寡醣與海帶岩藻寡醣於抗血小板凝集試驗上，濃度 5 mg/mL 與 20 mg/mL 之COS-3k 與 COS-DMF 最大凝集百分比為 21.5%-50.1%，濃度 5 mg/mL 與 20 mg/mL 之 FOS3k 與 FOS-3k-DMF，則為 84.1-121.4%。在Caco-2細胞模式中抗腸病毒 71 型活性部分，初步實驗結果顯示 100、10、和 1 microgram/mL 之 FOS3k 組於預防處理和共同處理皆可獲得較高之細胞存活率，分別為 88-98% 和 85-94%，皆高於控制組 (78%)，而 1 microgram/mL FOS3k-DMF 組則於治療處理得到最高細胞存活率 96%，較控制組 (53%) 高。 The aim of this study is to investigate the carrageenan-oligosaccharides and fucooligosaccharide FOS from Laminaria japonica. Activities of FOS as, prebiotic and antiplatelet aggregation, as well as to evaluate carrageenan-oligosaccharides and fucooligosaccharides on intestinal absorption and transport by intestinal model established with the human colon cancer cell line Caco-2 and the anti-enterovirus 71 strain test in Caco-2 cell model. The growth promoting effect of COS3k and COSDMF were tested with Lb. acidophilus and Lb. plantarum. The results showed that COS3k and COSDMF could show significant growth effect while compare to the growth of control group. FOS3k or FOS3k-DMF showed that while 1% FOS3k and FOS3k-DMF used as a carbon source, the viable counts of B. bifidum, B. adolescenis, Lb. acidophilus, and Lb. plantarum were significantly higher then control group had. The percentage of absorption and transportation of COS3k by Caco-2 were approximately 18.06% and 3.46%. in the 270 min, the amount of FOS3k and FOS3k-DMF absorbed by Caco-2 cells were 2.08 and 1.49 mg/mL, respectively. After 270 min reacting with Caco-2 cell, the percentages of absorption and transportation of FOS3k and FOS3k-DMF were 27.37% and 20.52% as well as 19.07% and 16.92%, respectively. The concentration of 5 mg/mL and 20 mg/mL of carrageenan-oligosaccharides and fucooligosaccharide can be reduced 21.5%-50.1% and 84.1-121.4% of platelet aggregation. The antivirus activity against enterovirus virus 71 strain in Caco-2 cell model experiments, the results showed that 100, 10, and 1 microgram/mL FOS3k performed inhibition activity at prevention assay and co-treatment assay, cell viability were 88-98% and 85-94% while compared to cell viability of control group 78%. And the highest cell viability was 96% by post-infection of fucooligosaccharide FOS3k-DMF in the concentration 1 microgram/mL

DSpace CRIS

Study on the Activity of Anti-eterovirus and Application as Prebiotics of Oligosaccharides from Carrageenan and Fucoidan

基本資料

Description