The Study on the Processing Protocol Based on Biorefinery Concept to Produce Pe and Ace Inhibitory Peptides from Porphyra Sp. and Using Seaweed Residues to Output Health Additive of Animal Feed and Make Phenyllactic Acid via Hydrolysis and Lab Fermentation(1/2)

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基本資料

Project title

Code/計畫編號

MOST108-2221-E019-039-MY2

Translated Name/計畫中文名

自紫菜以生物精煉概念生產藻紅蛋白(PE)與具ACEI活性胜肽及藻渣水解液經乳酸菌發酵產製苯乳酸(Ph-LA)與寵物飼料保健添加物之製程探討(1/2)

Project Coordinator/計畫主持人

Chorng-Liang Pan

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13128061

Year

2019

Start date/計畫起

01-08-2019

Expected Completion/計畫迄

31-07-2020

Bugetid/研究經費

1134千元

ResearchField/研究領域

食品科技(工)

Description

Abstract

本研究計畫旨在自紫菜生產藻紅蛋白(PE)與具血管收縮素轉化酶 (ACE)活性抑制胜肽，以及以生物精煉概念製程將藻渣經水解和乳酸菌發酵產製苯乳酸(Ph-LA)並探討做為動物飼料保健添加物效果。第一年的實驗工作包括: (1) 市售紫菜以海洋細菌 (MA103/MAEF108)誘導粗酵素液水解反應條件探討，(2) 以不同方法分離萃取紫菜藻紅蛋白(PE)之收率比較，(3) 萃取藻紅蛋白(PE)後之紫菜水解液及其乳酸發酵產物之ACEI活性胜肽之活性測試，(4) 紫菜藻渣單一或組合後做為動物飼料保健功能性添加物之可行性探討: 經口投予紫菜藻渣單一或組合配方是否調節小鼠免疫功能。第二年的實驗工作包括: (1) 自海藻和藻渣蛋白質產出可抑制ACE酵素活性之胜肽鑑定，(2) 紫菜藻渣水解液以乳酸菌生產苯乳酸之發酵條件探討，(3) 各階段產出之紫菜藻渣單一或組合後做為動物飼料保健功能性添加物之可行性探討: 經口投予紫菜藻渣單一或組合配方是否減緩小鼠食物過敏及遲發型過敏反應。期待可以建立紫菜與其他海藻以生物精煉概念之新穎與可再生之生物利用產業。 The aim of this research project is to develop the production technology of phycoerythrin and angiotensin I converting enzyme (ACE) inhibiting peptide from Porphyra, in addition, based on biorefinery concept to using the seaweed residues to make phenyllactic acid (Ph-LA) by further hydrolysis and lactic acid bacterial fermentation and to evaluate if the seaweed residues could be an effective animal health feed additive. In the first year, planed works including: (1) investigate the enzyme profiles that as marine bacteria strains MA103 and MAEF108 are induced by Porphyra, (2) using various methods to separate and extract the phycoerythrin from Porphyra, (3) employing the seaweed hydrolysate to recover the ACE-I peptides, and (4) testing the seaweed residue for understanding if it could regulate the immune system of test animal (BALB/c mouse). In the second year, planed works including: (1)the identification of ACE-I active peptide identified in the first year, (2) further htdrolysis of seaweed residue and fermentation by specific LAB strains to produce phenyllactic acid (as a novel niopreservative), (3) examing the seaweed residue to aware if it could exihibit the attenuation of food allergy and delayed-type hypersensitivity reactions on the test animal(BALB/c mouse). The experimental results will promote the novel and renewable bio-industry of Porphyra and other seaweed based on the biorefinery concept.

Keyword(s)

紫菜
酵素輔助水解
生物精煉
藻紅蛋白(PE)
血管收縮素轉化酶 (ACE)活性抑制胜肽
藻渣
動物飼料保健添加物
乳酸菌
苯乳酸 (Ph-LA) Porohyra
Enzyme aided hydrolysis
Biorefinery
Phycoerythrin
Angiotensin I converting enzyme (ACE)
Seaweed residue
Animal health feed additive
Lactic acid bacteria (LAB)
Phenyllactic acid (Ph-LA)