Production and Purification of Antihyperglycemic Substances from Hydrolysates of Kelp (Brown Alga) (Laminaria japonica)

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Project title

Code/計畫編號

NSC102-2313-B019-016

Translated Name/計畫中文名

海帶水解物中具降血糖生理活性物質的生產與純化技術開發

Project Coordinator/計畫主持人

Jenn-Shou Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=3086390

Year

2013

Start date/計畫起

01-08-2013

Expected Completion/計畫迄

01-07-2014

Bugetid/研究經費

820千元

ResearchField/研究領域

食品科技(農)

"海帶(Laminaria japonica)水解物，對糖解作用中的 α-glucosidase 與 α-amylase 活性具抑制作用，可延緩糖類在體內的吸收，進而降低餐後血糖濃度。同時，水解物中的活性胜肽亦可抑制二肽基胜肽酶-4 (dipeptidyl-peptidase 4, DPP-4)的活性，以提高血液中的 GIP(Glucose-dependent insulinotropic polypeptide 與 GLP-1 (glucagon-like peptide-1)的活性，進而增加胰島素分泌達到降低血糖的濃度。本研究擬選用海帶（Laminaria japonica）利用纖維素酶與蛋白酶進行水解，而製得具有降血糖（以胜肽類為主）與降血脂(水可溶性與不可溶性膳食纖維為主)之活性物質。因此，本研究擬以二年期間分別進行，第一年探討海帶水解物對 α-glucosidase、 α-amylase 與 DPP-4 抑制活性的影響，其中包含不同纖維素酶與蛋白酶的添加量與水解時間的選擇，另將海帶殘渣 (水可與不可溶的部分) 以模擬人體腸胃道系統對膽酸結合能力之影響(降血脂)；第二年則探討對具有抑制 α-glucosidase、α-amylase 與 DPP-4 活性之胜肽物質進行分離、純化與序列鑑定，以評估其海帶對降血糖之生理效果，並建立此保健食品作為產品開發之參考數據。" " Kelp (Laminaria japonica) hydrolysates have the inhibitory effect on α-glucosidase and α-amylase activiy to delay the absorption of glucose, that decrease the level of postprandial blood glucose. On the other hand, the bioactive peptides from hydrolysates could inhibit the dipeptidyl-peptidase-4 (DPP-4) activity to increase the bioactivities of GIP (Glucose- dependent insulinotropic polypeptide) and GLP-1 (glucagon-like peptide-1) in the plasma. Thus, the glucose-induced insulin secretion will be augmented, then, decreased the plasma glucose level. This study will be completed in two years to work on kelp (Brown seaweed) as materials. First year, the inhibitory effect on α-glucosidase, α-amylase and DPP-4 activity by kelp hydrolysates will be investigated, include the selection of different cellulase and proteases, and hydrolysis condition. In vitro of bile acid binding capacity of soluble and insoluble dietary fiber from hydrolysates will also be determined. Second year, the inhibitory peptides were further separated (gel filtration) and purified by reverse-phase HPLC and identified its sequences to evaluate the physiological effects on reducing blood glucose. From this study, the basic information for development of health food on blood glucose regulation and hypocholesterolemic effect will be established."

Keyword(s)

海帶
水解物
α-葡萄糖苷酶
α-澱粉酶
二肽基胜肽酶-4
調節血糖
Kelp
Hydrolysate
α-glucosidase
α-amylase
dipepidy-peptidase 4
Blood glucose regulation

DSpace CRIS

Production and Purification of Antihyperglycemic Substances from Hydrolysates of Kelp (Brown Alga) (Laminaria japonica)

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