Delivery of Stromal Cell Derived Factor-1 from Novel Fucoidan Nanoparticles in Stem Cell Therapy

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Project title

Code/計畫編號

NSC101-2221-E019-026

Translated Name/計畫中文名

新穎性褐藻糖膠奈米粒釋放基質細胞衍生因子應用於幹細胞療法

Project Coordinator/計畫主持人

Yi-Cheng Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=2583298

Year

2012

Start date/計畫起

01-08-2012

Expected Completion/計畫迄

01-07-2013

Bugetid/研究經費

920千元

ResearchField/研究領域

醫學工程
生物技術(醫)

"本計劃書擬以萃取自褐藻的褐藻糖膠(Fucoidan)製備奈米載體，控制釋放基質細胞衍生因子(Stromal cell-derived factor-1; SDF-1)，應用於組織工程。申請者藉由前一年的國科會補助計畫：從幾丁聚醣奈米粒釋放溶疤酶及生長因子應用於脊髓半斷靈長類動物之研究(NSC98-2320-B-019-001-MY3)，得到令人雀躍的結果，已發表在國際期刊Acta Biomaterialia 及Carbohydrate polymers，後續的實驗仍持續進行，成果亦持續發表中。過去一系列的研究著重於幾丁聚醣的應用，幾丁聚醣取自於海洋，因而想探討其他海洋資源在生醫材料利用的可行性，並結合控制釋放系統的概念，加速組織的修復。當組織受損時，會分泌特定化學因子吸引幹細胞遷移至該處進行組織修復。SDF-1為缺血性組織損傷之重要修復因子，在受傷組織大量釋出，能調控幹細胞的行為，藉由其對應之接受器CXCR4引發細胞進行訊息傳遞，介導細胞生長、遷移與分化之表現，然而SDF-1半衰期短，易受酵素降解，無法長時間誘導幹細胞遷移至損傷處，因此希望藉由控制釋放SDF-1以提高組織修復之成效。褐藻糖膠(Fucoidan)含有帶負電之硫酸基團，經研究指出具有抗發炎、抗病毒、抗凝血、調控生長因子等生理活性；幾丁聚醣(Chitosan)為帶正電的生物聚合物，已知具有多種生物活性與良好的生物相容性。本研究將以褐藻糖膠與幾丁聚醣製備奈米粒，控制釋放SDF-1，以保護並延長SDF-1之活性，調控間葉質幹細胞的遷移及分化，以增進幹細胞療法的成效。此外，亦期待對於海洋資源的開發及利用，提供一個新的方向。" "The plan is intended to fabricate fucoidan-based nanoparticles as carriers to delivery stromal cell-derived factor-1 (SDF-1) in tissue engineering. By the National Science Council grant program (Sustained release of chondroitinase and growth factors from chitosan nanoparticles for repairing the hemisection spinal cord injured primate, NSC98-2320-B-019-001-MY3), we got exciting results which has been published in the international journal “Acta Biomaterialia” and “Carbohydrate Polymers”. Follow-up experiments are still ongoing, and the results continue to publish. Series of studies over the past focused on the application of chitosan which comes from ocean. Therefore, I would like to explore other marine resources for the feasibility of biomaterials. Then, combine marine resources with the concept of controlled release system to improve tissue repair Recent studies indicate that stem cells can be attracted to injury sites by specific chemokines secreted by damaged tissue, and then promote tissue repair. One of the chemokines secreted is stromal cell-derived factor-1 (SDF-1). SDF-1 expression has been shown to increase under ischemic conditions and thus may serve to attract stem cells to sites of injury. SDF-1 binding to its cognate receptor, CXCR4, regulates a variety of cellular functions such as stem cell proliferation, migration, and differentiation. Despite its vital role, the use of SDF-1 chemokine in tissue regeneration is limited by its relatively short half-life and enzymatic degradation. Thus, delivery systems that sustained release SDF-1 can significantly improve the efficacy of SDF-1 in tissue engineering applications. Fucoidan, extracted from brown alage, is a polysaccharide with sulfated groups and has bioactivities including anti-inflammatory, anti-virus, anti-tumor, and anticoagulant. Chitosan, a cationic biopolymer, has known with good bioactivity and biocompatibility. The aim of this project is controlled release of SDF-1 from fucoidan/chitosan nanoparticles to protect and extend SDF-1 bioactivities and to induce long-time stem cells homing effect. We expected that this controlled release system could achieve the purpose of effective stem cell therapy and provide a new direction for the development and use of marine resources."

Keyword(s)

褐藻糖膠
幾丁聚醣
奈米粒
基質細胞衍生因子
幹細胞
Fucoidan
Chitosan
Nanoparticle
Stromal cell-derived factor-1
Stem cell

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Delivery of Stromal Cell Derived Factor-1 from Novel Fucoidan Nanoparticles in Stem Cell Therapy

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