Cross-Linked Ulvan Membranes as New Biomaterials for Wound Healing(3/3)

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基本資料

Project title

Code/計畫編號

MOST107-2314-B019-001-MY3

Translated Name/計畫中文名

以交聯之石蓴多醣薄膜為新穎生醫材料應用於傷口修復(3/3)

Project Coordinator/計畫主持人

Yi-Cheng Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13319569

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-12-2021

Bugetid/研究經費

1086千元

ResearchField/研究領域

其他（醫）

本計畫的目標是以萃取自綠藻的石蓴多醣製成薄膜，再利用交聯改善薄膜的特性，評估其做為傷口敷料的潛力。臺灣周圍海域富含藻類資源，卻常因其過度繁殖破壞海洋生態。藻類中存在獨特的硫酸多醣，已知具有抗氧化、抗發炎、抗病毒和抗腫瘤等多種生理活性；此外，其含量豐富、生物相容性高且具生物可降解性，近年來愈來愈多研究探討其發展為生物醫學材料的潛力。石蓴多醣(ulvan)為萃取自綠藻的天然多醣，其富含硫酸多醣和鼠李醣。硫酸多醣在傷口修復過程扮演重要的角色，可促進細胞增殖、抑制凝血酶生成和改善纖維蛋白溶解能力；富含鼠李醣之多醣類已知具有抗發炎、降低細菌貼附和防止紫外線損傷的能力，可刺激細胞增值和膠原蛋白合成，然而，由於石蓴多醣結構中含有硫酸基、羧基及羥基，具很強的吸水及膨潤特性，以致機械強度明顯不足，限制其在生物醫學上的應用。本計畫以環氧化物(epoxide)及帶正電的生物聚合物為交聯劑，利用化學共價交聯及離子交聯的方式，改善石蓴多醣薄膜的機械強度，應用於傷口修復。此三年計畫，第一年為石蓴多醣的萃取並分析其組成及性質；第二年為交聯之石蓴多醣薄膜的製備及性質分析；第三年為以大鼠傷口損傷的動物實驗模式，評估石蓴多醣薄膜為傷口敷料的潛力。 The aim of this proposal is to fabricate ulvan membrane, to improve its physical-chemical properties by cross-linking and then evaluate its potential as a wound dressing. Taiwan is rich in algae sources. However, algae are often involved in uncontrolled proliferation processes detrimental for marine and aquatic environments. A very intriguing feature that distinguishes algal biomass from other resources is that it contains large amounts of sulphated polysaccharides, which have been known to exhibit anti-oxidant, anti-inflammatory, anti-virus, and anti-tumor bioactivities. Moreover, algal polysaccharides are gaining particular attention for using as a biomaterial due to their abundance, compatibility, and biodegradability. Ulvan, a nature polysaccharide extracted from green algae, is rich in sulphated polysaccharide and rhamnose. Sulphated polysaccharide plays a pivotal role in the wound healing process by promoting fibroblast proliferation, inhibition of thrombin generation, or improvement of fibrinolytic functions. Rhamnose-rich polysaccharides demonstrate anti-inflammatory property, diminish skin bacterial adhesion, protect skin from UV-induced injure and stimulate cellular proliferation and collagen biosynthesis. However, ulvan is characterized by poor mechanical property because of impressive water uptake and swelling, resulting in its limitation on biomedical applications. This proposal tries to improve mechanical property of ulvan membrane by chemical covalent crosslinking and ionic crosslinking methods using epoxides and positively charged nature polysaccharides. This project includes three-year research program. The 1st year: extraction and characterization of ulvan. The 2nd year: preparation and characterization of crosslinked ulvan membrane. The 3rd year: evaluate the possibility of using ulvan membrane as a wound dressing using wound healing animal model of SD (Sprague Dawley) rat.

Keyword(s)

石蓴多醣
硫酸多醣
交聯
傷口修復
ulvan
sulphated polysaccharide
cross-linking
wound healing

DSpace CRIS

Cross-Linked Ulvan Membranes as New Biomaterials for Wound Healing(3/3)

基本資料

Description