Study on Mold Antigens---Roles of Peptidylgroups in the Antigens from Penicillium and Aspergillus

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Project title

Code/計畫編號

NSC82-0409-B019-003

Translated Name/計畫中文名

黴菌抗原分子之研究：Penicillium和 Aspergillus抗原分子中胜太群的角色

Project Coordinator/計畫主持人

Guo-Jane Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=48397

Year

1993

Start date/計畫起

01-08-1992

Expected Completion/計畫迄

01-07-1993

Bugetid/研究經費

694千元

ResearchField/研究領域

生物科學

Aspergillus parasiticus菌絲抗原萃取液經離子交換管柱Q cartrige及Con A親和性管柱分離,可得到具Con A親和性(BF)及不具Con A親和性(UBF)之抗原,其蛋白質與總醣之比值分別為0.51與2.51。Penicillium citrinum菌絲抗原經同樣處理,卻只能得到具Con A親和性之抗原,其蛋白質與總醣之比值為0.58。由加熱試驗顯示,在60.degree.C加熱1小時,三者抗原均很安定,但在100.degree.C加熱,則其活性迅速降低,尤以A. parasiticus之UBF抗原降低程度最大。以蛋白質分解酵素Pronase、Trypsin及Chymotrypsin分別作用於各分離之抗原,顯示測試之抗原均因Pronase之作用而降低其ELISA吸光值,尤以A. parasiticus之UBF下降程度為最大。Trypsin對抗原之影響與Pronase作用結果相似;但A. parasiticus之UBF及BF受Chymotrypsin作用而降低之ELISA吸光值卻遠低於P. citrinum之抗原之下降程度。.beta.-galactosidase可降低三種測試抗原之ELISA吸光值,但降低至某一程度後,即不再下降。.alpha.-amylase之作用亦可降低A. parasiticus UBF及P. citrinum抗原活性,但對A. parasiticus BF則不但未降低,反而可稍增加其ELISA之吸光值。經由SDS-PAGE膠電泳圖顯示,A. parasiticus之BF抗原和P. citrinum抗原之分子量分布非常相似,但A. parasiticus之UBF抗原卻與前二者明顯不同。由Western blotting試驗顯示,造成二菌抗原-抗體間交叉反應的抗原,為具有Con A親和性之抗原。 The antigens separated by Q cartridge and concanavalin A-Sepharose (Con A) column from Aspergillus parasiticus mycelium showed different Con A-binding characteristics: one with Con A-binding ability (BF), the other without Con A-binding ability (UBF); the ratios of protein to total sugar for these antigens were 0.51 and 2.51 respectively. However, only Con A-binding antigen can be obtained for Penicillium citrinum after following the same separation procedures and the protein/total sugar ratio is 0.58. All of these antigens were stable after the heat treatment at 60.degree.C for 1h; however, their ELISA absorbance values were decreased significantly after heat treatment at 100.degree.C for 5 min; especially the UBF from A. parasiticus. The ability to bind antibodies was markedly reduced by treatment of these antigens with pronase, trypsin, chymotrypsin and .beta.-galactosidase, with exception to chymotrypsin on A. parasiticus antigens. .alpha.-amylase also significantly reduced the antibody-binding abilities of A. parasiticus UBF antigens and P. citrinum antigens; but, it caused the increase of the ability to bind antibodies for A. parasiticus BF antigens. The molecular sizes of A. parasiticus BF antigens and P. citrinum antigens were similar, but significantly different from those of A. parasiticus UBF antigens after analyses by SDS-PAGE. The antigens with Con A-binding ability can cause the antibody-antigen cross reactions between Aspergillus and Penicillium species, as shown by Western blotting techniques.

Keyword(s)

寄生麴黴
抗原
酵素關連免疫吸附法
交叉反應
Aspergillus parasiticus
Antigen
Enzyme-linked immunosorbent assay (ELISA)
Cross reaction

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Study on Mold Antigens---Roles of Peptidylgroups in the Antigens from Penicillium and Aspergillus

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