Degradation of anti-nutritional factors in soybean meal by lactic acid bacteria using a novel solid-state fermentor( II )

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Project title

Code/計畫編號

MOST105-2622-E019-002-CC2

Translated Name/計畫中文名

利用新穎固態發酵槽操作系統以乳酸菌降解豆粕抗營養因子( II )

Project Coordinator/計畫主持人

Guo-Jane Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=11773164

Year

2016

Start date/計畫起

01-02-2016

Expected Completion/計畫迄

01-01-2017

Bugetid/研究經費

1068千元

ResearchField/研究領域

食品科技(工)

"豆粕為畜產飼料蛋白主要來源。近年來因魚粉價格上升，豆粕成為水產動物飼料之潛在蛋白來源。然豆粕甲硫胺酸及離胺酸含量低，且含植酸、皂素、胰蛋白酶抑制劑等抗營養因子，會降低動物之生長速率及生長表現，其中以植酸最受重視。雖有許多研究探討微生物植酸酶酵素特性，然實際將之用於固態發酵豆粕，以降解豆粕植酸之研究有限；且多以靜置方式進行固態發酵降解植酸，效果有限。利政科技公司最近設計開發一種具電腦程式化自動控制系統之新型攪拌方式之新穎固態發酵槽，除具特殊設計攪拌軸葉片外，外加槽體旋轉設計，可增加混合效率。乳酸菌為益生菌，死菌體仍有益動物體，且菌體蛋白可增加豆粕甲硫胺酸及離胺酸含量。本研究將以具植酸分解力的乳酸菌利用新穎固態發酵槽發酵豆粕，並與傳統瓶裝式固態發酵法比較，探討降解豆粕中植酸效果，另外，同時分析皂素及胰蛋白酶抑制劑的降解量。本研究第一年已完成「具植酸分解能力之乳酸菌篩選及其於三角瓶固態發酵降解豆粕/黃豆植酸」之探討。從市售可食魚種腸道之 25 株乳酸菌分離株，篩選出高植酸酶活性、且降解豆粕/黃豆植酸效果最好的乳酸菌 FPS 2520，建立其三角瓶之靜置固態降解豆粕/黃豆植酸之條件；並以二階段溫度固態發酵模式 (37℃ 培養24 hr ，升溫至50℃再培養24 hr)，可降解黃豆植酸降解率83%。第二年為「利用新穎性固態發酵槽之操控系統，進行乳酸菌降解豆粕植酸與組成分析」。先以固態發酵槽在 37℃下探討槽體旋轉、攪拌速度與通氣量對 FPS 2520在豆粕中生長與降解植酸的影響。接著在所建立槽體旋轉角度、攪拌轉率、及通氣量條件下，探討二階段發酵溫度降解植酸之效果。最後分析所得產物組成份、植酸及其它抗營養因子含量。同時，此數據可和第一年以三角瓶進行之靜置固態發酵方式所得比較。" "Soybean meal (SBM) is the major protein source for animal feed. The price of fish meal is raised up recently. People search for SBM as a potential to replace fish meal. However, there are two drawbacks of the lack of methionine and lysine in SBM protein, and the presence of antinutritional factors including phytic acid, saponin, and trypsin inhibitor, especially the phytic acid, in SBM that will retard the animal growth and affect the animal physiology. The characteristics of phytase from various microbial sources have been studied. However, only limited people investigate the phytate degradation efficacy by using solid-state fermentation (SSF) of SBM. In addition, people can only do SSF by adding SBM in container because lack of SSF equipment. Consequently, the phytate degradation efficacy was not good. A novel computer-programed SSF fermentor is newly developed by Li-Cheng Co. A special stirred fan is constructed inside the container and the container can be rotated, both of which increase the mixing effect that will significantly increase the phytate degradation efficacy. Lactic acid bacteria (LAB), the probiotics, irrespective live or dead, are favor for animal health. Due to the presence of LAB higher amounts of methionine and lysine are observed in LAB fermented SBM. The aims of this project are to use LAB with phytase activity to degrade phytic acid in SBM using the novel SSF fermentor. At the same time the contents of saponin and trypsin inhibitor in SBM should also be decreased. In the first year, the LAB strain of FPS 2520, with the highest pyhtase activity and phytate degradation efficacy in soybean was screened out from 25 LAB isolates from fish intestines. The phytate degradation conditions by FPS 2520 in soybean/SBM using settling flask-type fermentation were established. Then, the phytate degradation efficacy was further increased to 83% using 2-stage temperature fermentation. In the second year the phytate degradation efficacy will be evaluated using a novel SSF fermentor by this FPS 2520. The effects of the individual factors of rotation speed of fan and container, type and amount of air on the phytate degradation will be investigated at first. Then, at the optimal operating conditions obtained the phytate degradation efficacy in SBM by this strain using the 2-stage fermentation temperature will be evaluated. Finally, the composition, and contents of phytate and other antinutrients in the fermented SBM product will be analyzed."

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Degradation of anti-nutritional factors in soybean meal by lactic acid bacteria using a novel solid-state fermentor( II )

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