Development of Biotechnologic Identification to Differentiate the Puffer Products from Other High Economic Fish Products (I)

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簡歷

基本資料

Project title

Code/計畫編號

NSC92-2313-B019-020

Translated Name/計畫中文名

開發防制有毒河魨產品假偽高級魚種產品之生技鑑別技術(I)

Project Coordinator/計畫主持人

Deng-Fwu Hwang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=848624

Year

2003

Start date/計畫起

01-08-2003

Expected Completion/計畫迄

01-07-2004

Bugetid/研究經費

1005千元

ResearchField/研究領域

生物技術(農)
食品科技(農)

The aims of this work were to determine the differential characterization of the urea-soluble protein components of puffer fish species and to establish a preliminary proteomic database using immobilized pH gradient-two dimensional electrophoresis (2-DE) technique. The puffer fish muscle proteins resolved into 171-260 spots in the 2-DE gels, with a pI range of 3-10 and molecular weight range of 7.4-205.0 kD, following Comassie blue staining. Puffer fish muscle proteins fell in the region with pI values of 3.5-7.0 and molecular weights of 7.4-45.0 kD were well resolved and were good for species comparison. The more acidic proteins of lower molecular weights showed species-specific characteristics. Therefore, the species of puffer fish can be differentiated from the comparison of the characteristic 2-DE protein patterns.

Keyword(s)

two-dimensional electrophoresis
puffer fish
species identification
proteome
protein

DSpace CRIS

Development of Biotechnologic Identification to Differentiate the Puffer Products from Other High Economic Fish Products (I)

基本資料

Description