Studies on Distribution of Tetrodotoxin and Psp in Marine Biology, Control of Intoxification and Phylogenic Relationship of Species (III)

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基本資料

Project title

Code/計畫編號

NSC95-2313-B019-002

Translated Name/計畫中文名

河魨毒和麻痺性貝毒之生物種分布、毒化防制及生物種基因之相關性探討(III)

Project Coordinator/計畫主持人

Deng-Fwu Hwang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=1286769

Year

2006

Start date/計畫起

01-08-2006

Expected Completion/計畫迄

01-07-2007

Bugetid/研究經費

2050千元

ResearchField/研究領域

生物科學
基礎醫學

河魨毒(TTX)與麻痺性貝毒(PSP)為常見的兩種海洋生物毒，其作用機制為阻塞神經細胞上之鈉腔體，導致神經訊息無法傳遞。自九十年至九十三年間，在台灣共發生三起螺類中毒案件，經分析得知：榧螺科的橙口榧螺、台灣榧螺和平瀨榧螺、織紋螺科的球織紋螺、素面織紋螺和橡子織紋螺體內含有河魨毒，且並未檢出含有麻痺性貝毒，因此判定上述三起螺類中毒事件是由河魨毒所引起。截至目前，尚未開發出有效防治螺類蓄積毒素之方法，且對於中毒患者也無專一的治療藥劑可供使用，因此全面性的對台灣產螺類進行毒性市場調查，為防範螺類中毒事件的最佳方法之ㄧ。自民國九十一年至九十三年間，於台灣地區進行市場調查，於六個縣市中採集了十一種螺類，樣品數共一百七十粒。經毒性及毒成分分析得知，在嘉義及屏東地區所採集到的斑節楊桃螺及震旦海音螺含有河魨毒，毒素分佈於中腸腺及肌肉中。其餘的大玉螺、腰帶玉螺、條紋髻螺、花點鶉螺、鶴頭骨螺、花球琵琶螺、台灣鐘螺、千手玫瑰螺及赤蛙螺均未檢出含有毒性。根據有毒螺類毒性資料表得知，不同種螺類所含的毒素量差異甚大，此外少數螺類同時含有河魨毒及麻痺性貝毒。為了解不同種螺類毒性差異的原因，以及為何僅少數螺類可蓄積麻痺性貝毒，以建立河魨毒及麻痺性貝毒吸引性試驗。在河魨毒吸引性試驗中發現，有毒螺類的河魨毒相對吸引百分比與螺類的毒性呈現正相關 (Y=1.050X+3.443, R=0.8061)，並且螺類的毒性亦與其河魨毒耐受性呈現正線性關係(Y=0.113X+294.602, R=0.8143)。在麻痺性貝毒吸引性試驗中亦有相似的結果，有毒螺類的麻痺性貝毒相對吸引百分比與螺類的毒性呈現正相關(Y=3.716X+0.363, R=0.8427)，而螺類的毒性亦與其麻痺性貝毒耐受性呈現正線性關係(Y=0.091X+210.090, R=0.6803)。結果顯示毒性愈高的螺類愈受河魨毒所吸引，造成螺類毒性有高低差異，且麻痺性貝毒對有毒螺類也具有吸引力，使得部分螺類可同時蓄積兩種毒素。Tetrodotoxin (TTX) and paralytic shell poisons (PSP) are widely known marine neurotoxins, which act by blocking the entry of sodium ion into sodium channels of neuronal membranes. During 2001 to 2004, there were 3 gastropod poisoning incidents in Taiwan. Based on food poisoning incidents demonstrated that gastropods Olividae Oliva miniacea, O. mustelina, and O. hirasei, Nassariidae Nassarius glans, Zeuxis sufflatus, and Niotha clathrata contained TTX. While PSP was not detected in those causative gastropods, so it was concluded that the causative agent of the above gastropod poisoning incidents was TTX. Until now, it is no known strategy to prevent gastropods from accumulating the toxin and without a specific remedy for poisoning patients either. Therefore, the overall screening test of Taiwanese gastropods is the best way to prevent the happening of gastropod poisoning incident. During 2002 to 2004, 170 specimens of 11 species gastropods were collected from 6 prefectures in eastern, western and southern Taiwan. Both Harpa articularis and Hindsia sinensis collected from Chiay and Pingtung Prefecture were containing TTX. While, Polinices didyma, Natica vitellus, Phalium flammiferum, Tonna marginata, Murex haustellum, Ficus ficus, Chicoreus palmarosae, Calliostoma formosense and Bufonaria rana were not detected to contain TTX or PSP. TTX-bearing animals are generally known to be much more resistant to the lethal effect of TTX than TTX-lacking animals. In TTX attracting test, it was found that toxic gastropods showed significantly positive relationship between comparative attracting variation and the reported toxicity (Y=1.050X+3.443, R=0.8061), and the relationship between TTX resistance ability and the toxicity also has a positive correlation (Y=0.113X+294.602, R=0.8143). By the same way, in PSP attracting test, all toxic gastropods showed significantly positive linear relationship (Y=3.716X+0.363, R=0.8427) between comparative attracting variation and the toxicity reported. The relationship between PSP resistance ability and the toxicity also has a positive correlation (Y=0.091X+210.090, R=0.6803). It indicated that TTX and PSP are attractants for toxic gastropods.

Keyword(s)

河魨毒
麻痺性貝毒
吸引性試驗
Tetrodotoxin (TTX)
paralytic shell poisons (PSP)
attracting test

DSpace CRIS

Studies on Distribution of Tetrodotoxin and Psp in Marine Biology, Control of Intoxification and Phylogenic Relationship of Species (III)

基本資料

Description