Physiological Adaptations to Varying Nitrogen and Phosphorus Concentrations Regarding the Dominant Diatoms in Matsu Region: a Study of Molecular Mechanisms

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Project title

Code/計畫編號

MOST109-2611-M019-003

Translated Name/計畫中文名

在變動的氮磷濃度下馬祖海域優勢矽藻實行生理適應的分子機制

Project Coordinator/計畫主持人

Jeng Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=13546883

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

1905千元

ResearchField/研究領域

海洋科學

馬祖海域是富含營養鹽的水體，支持著以矽藻為起點的捕食食物鏈和各種漁業資源。由於營養鹽的濃度和比例控制了浮游植物的優勢種類，進而影響上層掠食生物的種類組成，所以計劃使用大規模定序技術在馬祖海域觀察優勢矽藻如何調控基因表現，藉以適應氮磷營養鹽的變動。申請人在2014至2019年之間持續參與馬祖航次，並採取浮游植物樣本進行添加氮磷的培養實驗。針對這些樣本，先後已經建構54個混和種類的全轉錄體 (metatranscriptomes)，但尚未分析各基因的mRNA表現量。由於骨藻 (Skeletonema spp.) 常常是樣本中的優勢矽藻，本申請案提議針對全轉錄體中的骨藻讀序進行分析，希望能解釋：(1) 在缺氮和缺磷狀態下有哪些生物化學的途徑受到影響，(2) 骨藻屬下的種類如何隨季節改變，(3) 在得自不同月份的全轉錄體中基因表現如何改變。在具體的工作內容方面，首先是補強現有的全轉錄體陣容，使得每一項營養鹽操控培養均有可供分析的全轉錄體。其次是進行特定種類的基因體定序，充實篩選骨藻序列時需用的模板資料庫。最後由全轉錄體中篩選出屬於骨藻的單一種類讀序，並利用適當的演算法進行校正和表現量差異的分析。本申請案對骨藻在馬祖海域的生理狀態進行全面性的評估，可以讓我們瞭解營養鹽如何影響群聚演替和優勢種的形成。 Matsu waters are rich in nutrients, which support a grazing food chain with diatoms as the major primary producers. Nutrient concentrations and stoichiometry are known to influence the dominant species in phytoplankton, and eventually determine the species composition of high-level predators. For this reason, this principal investigator (PI) proposes to investigate how dominant diatoms regulate gene expressions in response to changing nitrogen and phosphorus conditions in Matsu waters. The PI has conducted 12 sampling cruises between 2014 and 2019. Net-collected phytoplankton were incubated in media of various nitrogen and phosphorus concentrations. Using the nutrient-treated phytoplankton as raw materials, a total of 54 metatranscriptomes have been constructed, but a thorough analysis of gene expression has not been done. In this proposal, research efforts will be focused on Skeletonema spp., a dominant diatom genus frequently encountered in Matsu samples. By examining gene expressions, the following issues will be addressed: (1) Which biochemical pathways are affected in nitrogen and phosphorus deficiencies. (2) How species in Skeletonema genus change with seasons. (3) How genes differentially express in metatranscriptomes constructed in distinct months. As for experimental details, the PI will first construct more metatranscriptomes from nutrient-treated samples, which will create a complete sample series for further analysis. Next, genomic sequencing for specific Skeletonema spp. will be performed to establish a comprehensive database of DNA templates, which is necessary for selecting species-specific reads from metatranscriptomes. In addition, reads of target species will be filtered out, read-count normalization will be performed, and differential expressions will be identified using appropriate algorithms. This proposal will accomplish an overall evaluation of the physiological status for Skeletonema spp. in Matsu waters. Results of this study will lead to a better understanding of how nutrients influence diatom succession and formation of dominant species.

Keyword(s)

馬祖海域
浮游植物
矽藻
骨藻
營養鹽
大規模定序
高通量定序
生物化學途徑
轉錄體
全轉錄體
基因表現
Biochemical pathways
diatoms
gene expression
high-throughput sequencing
large-scale sequencing
Matsu waters
metatranscriptome
nutrients
phytoplankton
Skeletonema
transcriptome

DSpace CRIS

Physiological Adaptations to Varying Nitrogen and Phosphorus Concentrations Regarding the Dominant Diatoms in Matsu Region: a Study of Molecular Mechanisms

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