The Impact of Global Climate Change on the Energy Transference of the Microbial Food Web in the East China Sea and Kuroshio, Weastern Pacific- Ciliate Grazing

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Project title

Code/計畫編號

NSC101-2611-M019-015-MY3

Translated Name/計畫中文名

全球變遷對西北太平洋台灣海域海洋生物地球化學與生態系統之長期觀測與研究(I)-子計畫：全球變遷因子對東海及西太平洋黑潮海域微生物能量傳遞之影響-纖毛虫攝食

Project Coordinator/計畫主持人

Kuo-Ping Chiang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=2634462

Year

2012

Start date/計畫起

01-08-2012

Expected Completion/計畫迄

01-07-2013

Bugetid/研究經費

2033千元

ResearchField/研究領域

海洋科學

"由於全球氣候變遷對於海洋微生物生態系之影響所知非常有限，因此本計劃預計利用18S rDNA 進行超微真核生物與纖毛虫分類。一方面使用螢光原位雜合技術(FISH)與定量PCR (QPCR)進行超微真核生物定量，期望能了解這些超微真核生物與環境因子之關係。另一方面於東海海域進行少毛類纖毛虫生物多樣性之調查，預計針對少毛類纖毛虫數量及種類之時空分布做進一步的探討。針對纖毛虫與鞭毛虫進行分布及攝食研究，預期能在未來的實驗結果中看到鞭毛虫或者纖毛虫在不同環境之攝食率變化，並希望瞭解這些生物在不同水文環境下生長率和攝食率的差異，並掌握各別棲地微生物食物網之能量流動情況。本實驗藉由區分纖毛虫的種類和大小，能更精確得知生物在攝食上的反應與種類間的差異，本研究之成果與數據將能提供未來全球變遷研究之參考。" "Information on the influence of marine microbiologic ecology due to global climate change was rarely available. Our study, aiming to explore this problem, will examine the diversity of picoeukaryotists and ciliates by 18S rDNA sequence analysis, as well as the grazing rates of nanoflagellates by size fraction and of ciliates by fluorescence labeled particle method. The spatial and temporal distribution of picoeukaryotist abundance will be carried out by the FISH and QPCR to understand their distribution pattern and their relationship with environmental factors. For the ciliate diversity, we shall acquire more information about their morphology by protargol impregnation method. From the grazing experiment of nanoflagellates and ciliates, we shall obtain growth and grazing rates under different environmental conditions, understand the quantitative difference between these rates, and clarify the pathway of the energy flow in the microbial food web in different habitats of the East China Sea. In our grazing experiments on ciliates, we shall investigate the grazing response at species and size levels. Our result will be used as a data base for future study on the influence of global environmental change"

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The Impact of Global Climate Change on the Energy Transference of the Microbial Food Web in the East China Sea and Kuroshio, Weastern Pacific- Ciliate Grazing

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