Picoeukaryotes diversity and grazing rate in the subtroipcal marine coastal ecosystem

View Statistics Email Alert RSS Feed

Information

Details

Project title

Code/計畫編號

NSC101-2313-B019-004-MY3

Translated Name/計畫中文名

副熱帶沿岸生態系超微真核物多樣性與攝食率之研究

Project Coordinator/計畫主持人

Kuo-Ping Chiang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=2860273

Year

2013

Start date/計畫起

01-08-2013

Expected Completion/計畫迄

01-07-2014

Bugetid/研究經費

1500千元

ResearchField/研究領域

漁業

"微細真核生物是 pico 級浮游生物的主要攝食者，在微生物食物網中扮演著能量向上傳遞的重要的角色。這些pico 真核生物的組成會隨著全球暖化與海洋酸化而產生變化，這些變化會影響到微生物食物網之結構。先前已經針對台灣東北沿岸的pico 與nano 級生物在微生物食物網中的能量傳遞進行研究，但是對於超微真核生物的組成並不清楚。因此本計劃的目的是利用分子生物技術來瞭解台灣東北海域微細鞭毛蟲與超微真核生物之組成與生態研究，主要分為三部分：1)用18S rRNA 基因序列分析進行超微型真核生物之多樣性研究; 2)用螢光原位雜合反應法(FISH)進行混營性微細鞭毛蟲-著鞭毛藻之攝食率估算; 3) 用定量qPCR 法進行超微型真核藻類中青綠藻日夜數量變動之研究。以上研究有助於了解不同種類的真核生物在一個副熱帶沿岸生態系的微生物食物網所扮演的功能性角色。" "Picoeukaryotes are the major grazer on picoplankton, which plays an important role on the trophic link within microbial food web. Moreover, global warming and acidification could cause the rapid change in their species composition, which further affecting the structure of the microbial food web. Previous studies have investigated the carbon flow between pico- and nano- plankton in microbial food web of the northeast coastal ecosystem of Taiwan. However, it is rarely known about the grazing rate and diversity of picoeukaryote at species level. Therefore, the goal of this project is to apply molecular techniques to elucidate the composition of picoeukaryotes in three directions. 1) the diversity of the picoeukaryotes by 18S rRNA gene analysis; 2) the grazing rates of mixotrophic nanoflagellates, haptophytes by FISH method; 3) the dial variation of picoeukaryotic prasinophytes by qPCR. This study will strengthen our understanding of the functional role of different picoeukaryotes in the microbial food web in a subtropical coastal ecosystem."

DSpace CRIS

Picoeukaryotes diversity and grazing rate in the subtroipcal marine coastal ecosystem

Details

Description