The Impact of Global Climate Change on the Energy Transference of the Microbial Food Web: The Grazing Behaviour of Mixotrophic hypoyhyes

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Project title

Code/計畫編號

MOST104-2611-M019-022-MY3

Translated Name/計畫中文名

全球變遷對西北太平洋臺灣海域海洋生物地球化學與生態系統影響之長期觀測與研究(Ⅱ)---子計畫：全球變遷因子對東海及西北太平洋黑潮海域微生物能量傳遞之影響：混營性鞭毛蟲 (著鞭毛藻)攝食行為

Project Coordinator/計畫主持人

Kuo-Ping Chiang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=11578647

Year

2015

Start date/計畫起

01-08-2015

Expected Completion/計畫迄

01-07-2016

Bugetid/研究經費

2117千元

ResearchField/研究領域

海洋科學
大氣科學
自然生態保育

"微細鞭毛蟲(nanoflagellate)群集與纖毛蟲(ciliate)為picoplankton(細菌及藍綠細菌)主要的攝食者，在微生物食物網中具能量傳遞的重要的地位。其中混營性(mixotrophy)的微細鞭毛蟲具有兩套的營養方式(光合作用及攝食餌料行為)被指出在許多大洋環境中是為優勢種類，尤其是混營性的著鞭毛蟲(Haptophytes)所貢獻的基礎生產力及攝食控制力最被為重視。本計劃的目的是利用分子生物技術及大量定序來研究東海海域混營性著鞭毛蟲的數量分佈、種類組成與生態因子之間的關係，主要分為兩部分：1)以螢光原位雜合反應法(fluorescence in situ hybridization)進行混營性微細鞭毛蟲-著鞭毛藻之數量分佈及攝食率研究; 2)針對著鞭毛蟲的LSU rRNA 進行大量定序，研究在不同環境及時空下種類組成及其親緣關係。以上研究有助於更進一步的了解東海海域的優勢種類的攝食能量傳遞情況，並且亦可推估副熱帶生態系中混營性微細鞭毛蟲在海洋中的生態區位。另一方面進行東海海域纖毛蟲進行研究，對其數量及種類之時空分佈做探討，希以了解其多樣性及分佈情況。" "This project mainly investigates the environmental biodiversity and community composition of nanoflagellates and ciliates of the microbial food web in the East China Sea. Nanoflagellatesand ciliates control the abundance of picoplankton, including bacteria and Synechococcus, and are vitally influential to microbial food web. Mixotrophic nanoflagellates, which combine phototrophy and grazing behavior in one cell, are considered to be a dominate group in the marine environment. Recent studies have indicated that the Haptophytes (mixotrophy) are responsible for a large percentage of the total CO2 fixation and total bacterivory. Thus, our purpose is to study the variation of the diversity and species composition of Haptophytes related with biotic and abiotic environmental factors in the East China Sea, using molecular technique and pyrosequencing. This project shall pay special attention to two specific aspects: first, we shall investigate the diversity, distribution and ingestion rates of Haptophytes by fluorescence in situ hybridization; second, we shall use large subunit ribosomal RNA (LSU rRNA) fragments to assess the specie composition of Haptophytes in different spatial and temporal distribution by 454 pyrosequencing. We expect to make a significant progress in the investigation of the grazing behavior of the dominate Haptophytes species in the East China Sea. Furthermore, we shall demonstrate the niche of mixotrophic nanoflagellate in the subtropic marine ecosystem. This project also shall estimate the abundance and species composition of ciliates in spatial and temporal scales in the East China Sea. Our ultimate objective is to interpret the relationship between ciliate diversity and environmental factors."

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The Impact of Global Climate Change on the Energy Transference of the Microbial Food Web: The Grazing Behaviour of Mixotrophic hypoyhyes

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