Investigation on Co-Cultivation of Microalgae with Antrodia Camphorate for Enhancing Production Rate of Biodiesel and Added Value of Functional Components.-1

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Project title

Code/計畫編號

MOST105-2221-E019-066

Translated Name/計畫中文名

微藻與牛樟菌共同培養以提升微藻生質柴油產率與機能性成份附加價值之研究-1

Project Coordinator/計畫主持人

Hong-Ting Lin

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

林成原

Department/Unit

Department of Marine Engineering

Year

2016

Start date/計畫起

01-06-2016

Expected Completion/計畫迄

31-07-2017

Co-Investigator(s)

Cherng-Yuan Lin

Bugetid/研究經費

801千元

ResearchField/研究領域

能源工程

微藻油脂含量高且生長快速，被視為可大量取代化石燃料的可再生燃料的油脂來源，且微藻在增殖過程比陸基生物消耗較大量的二氧化碳作為成長碳源，因此可有效抑制溫室效應的上升。惟微藻於快速新陳代謝所釋放之廢棄物質於培育水體中持續累積，會破壞微藻的生長環境，而降低其繁殖速率及密度。牛樟菌含有特殊三萜類、多醣體、腺苷等高生物活性物質達78種以上，被認為具抗腫瘤、抗氧化、護肝…等功效，有臺灣紅寶石的稱號，已逐步應用於生技及醫療產業，經濟價值高。本計畫擬利用微藻與牛樟菌共同培養的方式，建立此二微生物的生態平衡及互利共生機制，以促進不同微生物的相互生長，提高微藻及牛樟菌的產率，以降低利用微藻油脂製造生質柴油成本，同時提高牛樟菌的經濟效益。共同培養的微藻株與牛樟菌將進行組成及性質分析，以篩選合適藻株，並建立最適化的高密度共同培養條件。本計畫規劃三年期程完成相關工作。第一年著重在篩選適合與牛樟菌共同培養的高油脂含量與高成長速率的藻株，並測試微藻油脂與牛樟菌的組成與性質，包括微藻生質柴油的燃料性質與牛樟菌的三萜類及多醣體等含量及性質。第二年強調建立最適化的微藻與牛樟菌共同培養條件，以促進微藻油脂產量及牛樟菌機能性成份生長的關鍵培養參數。第三年將綜合前二年的研究成果，調整微藻與牛樟菌的生長環境，以進一步建立提高此二微生物的成長密度，增加單位面積產率及品質的關鍵技術，並根據微藻油脂、牛樟菌機能性成份、生質柴油的燃料性質與引擎性能等各項性質的測試結果，適度調整製程參數，之後對於共同培養的微藻生質柴油進行生命週期分析，評估其能源輸出輸入比，以達到提高牛樟菌與微藻的產量及產值，降低製程生產成本，同時提昇經濟效益的目標。Microalgae bearing with high lipid content and fast growing rate is recognized as promising lipid feedstock of renewable fuel to replace fossil fuel. Moreover, microalgae will absorb much more carbon dioxide than any terrestrial plant during their proliferating process, leading to retardation of global warming effect. However, waste compounds through fast metabolism mechanism would accumulate in culturing water medium. The growing environment of microalgae could therefore be ruined and thus reduce their breeding rate and growing density. Antrodia camphorate which contains at least 78 high bio-activity compounds such as triterpene, polysaccharides and adenosine is found to have functions of anti-cancer, anti-oxidation and liver protection and thus owns high economic value. Co-cultivation of Antrodia camphorate with microalgae will be applied in this study in order to establish mechanisms of ecological balance and mutualism and thus facilitate the accretion with each other. The production rates of those two microorganisms would be enhanced together with reduction of production cost of microalgae biodiesel and meanwhile promotion of economical benefits of Antrodia camphorate. The compositions and properties of the co-cultured microalgae and Antrodia camphorate would be analyzed to screen adequate microalgae strain and build up the optimum co-culturing condition of high growing density. A three-year proposal is organized for this study. In the first years, the research will focus on screening microalgae strain with high lipid content and growing rate to co-cultivate with Antrodia camphorate. Chemical compositions and properties of those two microorganisms such as fuel properties of microalgae biodiesel and functional components of Antrodia camphorate. In the second year, the research will emphasize on establishing the co-cultivation condition and parameters to increase both the quantities of microalgae lipid and functional components. In the final years, the key technologies for enhancing high-densities of microalga and Antrodia camphorate with fast production rate and superior qualities will be developed. The compositions, properties, and function including engine performance will be analyzed. The co-culturing environment, growing conditions, and key technologies will be adjusted to optimize based on those test results. A life cycle assessment and energy yield ratio for the microalgae biodiesel will thereafter be carried out. As a consequence, the purpose of the increase of production quantities and economic benefits of both the microorganisms will be achieved.

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Investigation on Co-Cultivation of Microalgae with Antrodia Camphorate for Enhancing Production Rate of Biodiesel and Added Value of Functional Components.-1

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