Laser Desorption/Ionization Mass Spectrometry and Raman Spectrometry Coupled with Functional Nanoparticles for Detection of Pathogens and Tumor Cells and Imaging of Tumor Tissues(3/3)

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Project title

Translated Name/計畫中文名

雷射脫附游離質譜與拉曼光譜儀結合功能化奈米粒子於病原體與腫瘤標記物偵測與腫瘤組織顯影(3/3)

Project Coordinator/計畫主持人

Chih-Ching Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=13320323

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

3060千元

ResearchField/研究領域

化學

This project aims to utilized functional nanoparticle coupled with laser desorption/ionization mass spectrometry (LDI-MS) and Raman spectrometry applied to the detection of pathogens, cancer cells and tumor tissue imaging. Heteroatoms-doped carbon quantum dots (CQDs) will be synthesized through a simple one-step dry heating method of a carbon-rich material with an inorganic dopant. By altering the type of heteroatoms and composition in CQDs, we can synthesize CQDs employed for LDI-MS and Raman spectrometry applications. These CQDs can be coated onto gold nanoparticles through simple multivalent ligand–metal interaction to form carbon quantum dots coated metal nanoparticles (MNPs@CQDs). Recognition ligands may be functionalized onto MNPs@CQDs for specific targeting of pathogens, tumor markers, and tumor tissues. The unique structure of MNPs@CQDs allows it to be coupled with Raman and LDI-MS for further signal enhancement. The synergistic effect of the carbon shell and the metal nanoparticle core can increase the desorption/ionization efficiency and thereby increase the mass reporter signal. Additionally, the localized surface plasmon resonance (LSPR) effect of the metal core can act as Raman reporter amplifier and thus increase reporter CQDs sensitivity. Combined with immunostrip assays, MNPs@CQDs can be applied to clinical detection of pathogens and tumor markers with high speed and sensitivity. Furthermore, the dual modal detections allow sensitive detection of minute tumor tissue, which makes implementation of personal medication for cancer prevention therapy possible. 本計畫擬以雷射脫附游離質譜 (laser desorption/ionization mass spectrometry, LDI-MS)以及拉曼光譜儀 (Raman spectrometry)結合功能化奈米粒子應用於病原體與腫瘤標記物偵測以及腫瘤組織成像。我們將利用無機化合物與含碳物質一步混燒的方式，合成摻雜異原子的碳量子點 (carbon quantum dots, CQDs)。藉由改變異原子的種類以及摻雜於CQDs的比例，我們期望能合成同時具有LDI-MS以及Raman訊號的CQDs。另外利用簡單多配位 (multivalent)鍵結方式將CQDs修飾於金屬奈米粒子上，形成碳量子點-金屬複合粒子 (CQDs coated metal nanoparticle, MNP@CQDs)。將在CQDs表面修飾抗體和適合體等辨識分子，MNP@CQDs即可選擇性標定至病毒、腫瘤標記物以及腫瘤細胞，利用MNP@CQDs特殊結構能同時搭配LDI-MS以及Raman光譜來提升檢測訊號強度。MNP@CQDs在LDI-MS方面能利用CQDs和金屬奈米粒子的協同效應 (synergistic effect)提升雷射光吸收效率以及游離效率，在Raman光譜部分可以藉由金屬核的局域表面電漿共振 (localized surface plasmon resonance, LSPR)效應強化其拉曼散射。我們所開發MNP@CQDs結合簡單試紙檢測將可利用於臨床致病菌感染與腫瘤標記物快速和高靈敏診斷，落實個人醫療目的。而在組織顯影部分我們希望藉由LDI-MS和Raman光譜多重高感度檢測能發現極小腫瘤用於癌症預防治療。

Keyword(s)

Nanocomposites
Pathogens
Tumor markers
Tissue imaging
Laser desorption/ionization mass spectrometry
Raman Spectrometry
奈米複合物
病原體
腫瘤標記物
組織成像
雷射脫附游離質譜
拉曼光譜

DSpace CRIS

Laser Desorption/Ionization Mass Spectrometry and Raman Spectrometry Coupled with Functional Nanoparticles for Detection of Pathogens and Tumor Cells and Imaging of Tumor Tissues(3/3)

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