Studies on the immunity improvement and disease resistance by analyzing intestinal microbiota in the grouper and its application(IV)

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Project title

Code/計畫編號

MOST109-2321-B019-005

Translated Name/計畫中文名

以次世代定序技術開發水產生物健康腸胃道菌相生物製劑並增進其腸道免疫功能-提升石斑魚免疫力及抗病能力的腸道菌相確認及其應用(IV)

Co-Investigator(s)/共同執行人

吳金洌(計畫主持人)
白敦文

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=13568859

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Co-Investigator(s)

Pin-Wen Chiou
Li-Li Chen
Hsin-Yiu Chou

The aquaculture industry is one of the economically important sources in Taiwan. However, the aquatic animals were threatened by pathogen infections and thus cause huge losses during the process. Besides, the concerns on the impacts of global environmental changes on the pH and temperature of ocean waters have triggered enthusiasm in studying the influences of the changes on the wellbeing of marine animals. Environmental factors could affect the development, physiology and disease resistance, and ultimately the survival of animals in an altered environment. The purpose of this study is to analyze the gut microbiome in aquatic animals and create a non-toxic aquaculture environment by using natural, pre-existing microbiome as the probiotics to against disease and environmental stress; thus, they can grow naturally and reduce mortality without any drugs. Therefore, this project adopts an interdisciplinary approach, integrating researches in aquatic disease control and bioinformatics for further investigate the functional analysis of the potential probiotics and cooperate with biotechnology manufacturers to develop novel probiotic feed additives through large-scale production for commercialize. In addition, a class A CpG ODN molecule has been developed as a potent immunostimulants suitable for high water temperature. Based on the previous results, we seek to evaluate the efficacy of an anti-NNV vaccine containing the CpG ODN under different modes of high-water temperature. The development of such vaccine will impose great value to the grouper aquaculture industry to cope with the impact of ocean warming. Through these findings, it may help to reduce the usage of chemical agents and optimize the overall aquaculture environment to enhance the output value of aquaculture products. 水產養殖為台灣重要經濟產業，但養殖過程中容易遭受疾病嚴重感染，導致國內產量偏低。此外，全球暖化造成海水溫度劇度變化及酸化，其對於水生生物的影響遂成為刻不容緩的研究課題。環境因子改變會影響生物的發育、生理、及疾病抵抗力、終而該物種在變遷環境中的適應及生存能力。本研究的目的在利用天然的、已存在的腸道菌作為益生菌對抗疾病及環境逆境，達到不使用藥物亦可讓蝦類自然成長、減少死亡，塑造無毒養殖環境。因此本計畫將延續前三年跨領域結合水產疾病防治與生物資訊學研究，擬進一步確認益生菌的功能性分析，並與產商合作透過量產開發新的複合菌飼料添加物，之後針對不同魚蝦類進行現場應用，進而取代現場養殖過程中，預防性抗生素的不當使用。進一步評估添加class A CpG ODN的抗NNV病毒疫苗在不同高溫環境模式下對於石斑魚苗的保護效果，以發展功效不受高溫抑制的抗病毒疫苗，研發成果將具有對石斑魚產業因應海洋環境變遷的重要價值。除了協助國內水產養殖業者從源頭預防至養殖池自主健康管理，更希望所生產的優質本土水產品，能提高我國水產品於國際之競爭力和經濟效益。

Keyword(s)

養殖生物
腸道菌相
腸道免疫
益生菌
病原體
海水暖化
Aquatic animals
intestinal microbiota
intestine immunity
probiotic
pathogen
ocean warming

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Studies on the immunity improvement and disease resistance by analyzing intestinal microbiota in the grouper and its application(IV)

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