Separation and Purification of Collagen and Gelatin Hydrolysates from Skins of Tilapias (Oreochromis niloticus) and Study of Their Potential Biological Functionalities

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Project title

Code/計畫編號

MOST104-2311-B019-002

Translated Name/計畫中文名

吳郭魚魚皮膠原蛋白及明膠水解產物的分離純化與其生理功能性之研究

Project Coordinator/計畫主持人

Yu-Wei Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=11586918

Year

2015

Start date/計畫起

01-08-2015

Expected Completion/計畫迄

31-07-2016

Bugetid/研究經費

564千元

ResearchField/研究領域

食品科技(農)

養殖吳郭魚的加工產業中，特別是台灣鯛冷凍魚片的加工，常伴隨而來大量的加工副產品如魚皮、魚頭及魚骨，一直是低度利用之加工素材；然而這些原料富含大量的蛋白質，如魚鱗、魚皮中的膠原蛋白及附著於魚頭及魚骨的肌纖維蛋白等。透過吳郭魚皮的膠原蛋白萃取、蛋白質體鑑定及生物資訊工具的預測可得知，吳郭魚膠原蛋白吳郭魚膠原蛋白為多種活性胜肽之前驅物（活性胜肽的氨基酸序列的覆蓋率占總序列的89.7％），如抗氧化活性(AY、EL、WY、PHD、KD…)、抑制血管收縮轉換酵素活性(RL、IR…)、抗健忘活性(GPGG、PGP、PG、GP)、抗血栓活性(GPRG、GPR…)、調節免疫活性 (YG、GVM)、刺激葡萄糖吸收活性(VL、IV、II…)胜肽等，因此本計畫將著重於吳郭魚皮中膠原蛋白和明膠的萃取及利用，藉由適當的萃取、濃縮、水解、純化及分析鑑定的手段建立高品質標準生產流程，並將這些低度利用的蛋白質轉化成高經濟附加價值的活性胜肽產品或基材，同時並探討其具有潛力的生理活性功能，以期應用於食品、化妝及生醫產業。本計畫預計以兩年的時間達成下列目標: 第一年: 1. 建立吳郭魚皮之膠原蛋白及明膠分離物的萃取方法以提升其純度或產能，擬以不同酸(鹽酸、醋酸、檸檬酸)萃取、酸鹼pH轉移或結合高溫水解的技術去探討萃取的效率、產率及蛋白質純度的差異，進而建立標準化的優質萃取生產製程。 2. 根據27種常用蛋白脢，進行電腦篩選及預測最適合膠原蛋白及明膠的水解脢，以期生產具有特異性及不同效能之活性胜肽水解產物。 3. 執行in vitro酵素水解及生理活性實驗，生產具有不同生理活性之膠原蛋白及明膠水解產物，並利用蛋白質分析鑑定技術分離、純化水解產物。第二年: 4. 針對水解產物進行in vitro生理活性功能實驗，包括抗氧化活性、抑制血管收縮轉換酵素活性、抗健忘活性、抗血栓活性、抗菌活性試驗。 5. 進行膠原蛋白及明膠水解產物之體外消化及生理活性實驗調查，評估生產較適合人體消化吸收的活性胜肽前驅物。 Massive byproducts such as fish skin, heads and bones are produced from processing industrial of cultivated tilapias especially the processing of fillets from fresh water tilapias cultivated in Taiwan; generally these byproducts are underutilized processing materials which are relatively rich in proteins such as collagens from fish skin, and muscle proteins from residual flesh that are attached to fish bones and heads. According to the results from extraction and characterization of collagens, and prediction by bioinformatics tools, tilapias collagens contain precursors of multiple bioactive peptides such as antioxidant (AY、EL、WY、PHD、KD…), ACE inhibitor (RL、IR…), antiamnestic (GPGG、PGP、PG、GP), glucose uptake stimulating (VL、IV、II…), antithrombotic (GPRG、GPR…), immunomodulating activities (YG、GVM). Consequently, this project focuses on the utilization of tilapias collagens. With appropriate protein techniques of extraction, concentration, purification and identification, these proteins could be transformed to high value-added products of bioactive peptides or matrix applied in food, cosmetic and biomedical industrials. The following goals would be achieved in this two-year project: First year: 1. To establish standardized protocols of tilapias collagens and gelatins extraction methods for better yield and quality; this will be conducted by comparison of extraction efficiency using different acids (hydrochloric acid, acetic acid and citric acid), pH shift techniques and in combination of high temperature hydrolysis approach. 2. To simulate the enzyme action on protein sequences with 27 common proteases and to perform computational screening of optimal hydrolysates for tilapias collagens gelatins prior to produce specific bioactive peptide products with different potential biological activities. 3. To execute enzymatic hydrolysis in production of collagen and gelatin hydrolysates using protein identification and characterization techniques. Second year: 4. To implement biological activities in vitro experiments for enzymatic hydrolysates including antioxidant, ACE inhibitor, antiamnestic, antithrombotic and antibacterial activity assays. 5. To perform in vitro pepsin-pancreatin digestion with collagen and gelatin hydrolysates thereafter investigate their biological activities in assessment for precursors of bioactive peptides that are better suited for human digestion.

Keyword(s)

吳郭魚
膠原蛋白
明膠水解產物
活性胜肽
tilapias
collagen
gelatin hydrolysates
bioactive peptides

DSpace CRIS

Separation and Purification of Collagen and Gelatin Hydrolysates from Skins of Tilapias (Oreochromis niloticus) and Study of Their Potential Biological Functionalities

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