Preparation of Protein Isolates and Hydrolysates from Taiwan Local Macro-Algaes and Investigation of the Biological Activities of Their Bioactive Peptides

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Project title

Code/計畫編號

MOST106-2311-B019-001

Translated Name/計畫中文名

台灣本土巨藻之蛋白質分離物與水解物之製備與其活性胜肽之功能性探討

Project Coordinator/計畫主持人

Yu-Wei Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=12235766

Year

2017

Start date/計畫起

01-08-2017

Expected Completion/計畫迄

31-07-2018

Bugetid/研究經費

938千元

ResearchField/研究領域

食品科技(農)

台灣四面環海，海洋資源豐富，近年來藻類的利用備受重視，本計畫擬針對台灣本土巨藻中的野生綠藻(石蓴)、褐藻(馬尾藻)及養殖紅藻(海木耳)為原料，生產並開發藻類高蛋白質分離物與功能性水解產物，提高藻類蛋白質的附加價值及利用性。過去三年本實驗室已建立一套系統，以蛋白質體學為主軸，鑑定食品中的蛋白質序列，並利用生物資訊工具預測具有潛力的活性胜肽與水解酵素，後端以試管及動物實驗交叉驗證其水解物的生理活性，相關成果已於 J. Functional Food (2015) 發表，研究成果指出，吳郭魚魚皮膠原蛋白為多種活性胜肽之前驅物，同時本實驗室也對石蓴蛋白質進行活性預測，結果也指出藻蛋白也具有多種有潛力的功能性，特別是抑制血管收縮轉換酵素、抗健忘、抗血栓、降血糖及抗氧化活性，因此本計畫將延續此系統，將著重於藻類蛋白分離物和水解產物之活性胜肽的分離純化與其多重功能性驗證，先以in vitro 實驗進行活性評估後，再與加拿大合作實驗室進行in vivo 動物試驗，以原發性高血壓大鼠為動物實驗對象，各別探討活性胜肽(抑制ACE 活性)之降低大鼠血壓功效，期望在三年的計畫中，成功地開發出人類替代蛋白的來源之藻類蛋白分離物原料和具功能性的水解蛋白保健產品。 Taiwan is surrounded by oceans and abundant in seafood resources. Recently, the utilization of macro-algae is getting more attention. In order to level up the added value and to increase the utilization of macro-algae, this project is designed to use Taiwan locally wild green algae (Ulva lactuca), brown algae (Sargassum cristaefolium) and cultured red algae (Sarcodia montagneanat) to produce and develop algae protein isolates and functional protein hydrolysates. Over the past three years, our laboratory has established a system which is centralized by proteomics approach to identify food protein sequences and to apply the bioinformatics tools to predict potential bioactive peptides and suitable proteolytic enzymes. From this point onwards, the biological activities of protein hydrolysates were cross proofed by both in vitro and in vivo tests. According to our recent study published in Journal of Functional Foods, tilapias collagens contain precursors of multiple bioactive peptides. Meanwhile our laboratory analyzed the protein sequence from green algae (Ulva lactuca) and found that it had the potential to have multiple bioactive peptides as well in particular with ACE inhibitor, antiamnesic, antithrombotic and antioxidant activities. Consequently, this project will focus on the separation and purification of bioactive peptides from macro-algae and the assessment of their multi-functional activities. Firstly, the experiments will undergo in vitro evaluation on various activities. Following the in vitro results, in vivo experiments will be performed with our partner laboratory in Canada in order to evaluate the inhibition effects of purified bioactive peptides on hypotension effects using an animal model with spontaneous hypertension rats (SHR). Hopefully, the proposed three-year project will successfully produce and develop human substitute protein sources and functional protein hydrolysates with nutraceutical effects

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Preparation of Protein Isolates and Hydrolysates from Taiwan Local Macro-Algaes and Investigation of the Biological Activities of Their Bioactive Peptides

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