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http://scholars.ntou.edu.tw/handle/123456789/20306
2024-03-27T08:17:25ZAluminum (Al) causes a delayed suppression of nucleotide excision repair (NER) capacity in zebrafish (Danio rerio) embryos via disturbance of DNA lesion detection
http://scholars.ntou.edu.tw/handle/123456789/22206
標題: Aluminum (Al) causes a delayed suppression of nucleotide excision repair (NER) capacity in zebrafish (Danio rerio) embryos via disturbance of DNA lesion detection
作者: Paul, Ganjai Vikram; Huang, Ya-Yun; Wu, Yu-Ning; Ho, Tsung-Nan; Hsiao, Hsin-I.; Hsu, Todd
摘要: Aluminum (Al) is extensively used for making cooking utensils and its presence in the aquatic environment may occur through acid mine drainage and wastewater discharge. Al is known to induce genotoxicity in human cells, rodents, and fish. Nucleotide excision repair (NER) eliminates helix-twisting DNA lesions such as UV-induced dipyrimidine photoproducts. Because our earlier investigation revealed the operation of NER in zebrafish (Danio rerio) embryos, this study explored if inhibition of NER could be a mechanism of Al-induced genotoxicity using zebrafish embryo as a model system. An acute fish embryo toxicity test indicated that Al (as aluminum sulfate) at 2-15 mg/L were nonlethal to zebrafish embryos, yet exposure of embryos at 1 h post fertilization (hpf) to Al at 10-15 mg/L for 71 h significantly repressed their NER capacity monitored by a transcription-based DNA repair assay. Band shift analysis indicated a higher sensitivity of (6-4) photoproduct (6-4PP) than cyclobutane pyrimidine dimer (CPD) detecting activities to Al, reflecting the preferential influence of Al on the detection of strongly distorted DNA lesions. Time-course experiments showed a delayed response of NER to Al as repair machinery was unaffected by Al at 15 mg/L following a 35-h exposure, while Al treatment for the same period obviously inhibited 6-4PP binding activities although the gene expression of damage recognition factors remained active. Inhibition of 6-4PP detection blocked downstream lesion incision/excision detected by a ter-minal deoxy transferase-mediated end labeling assay. As the disturbance of damage sensing preceded that of the overall repair process, Al exposure was believed to downregulate NER capacity by inhibiting the activities of lesion detection proteins. Our results revealed the ability of Al to enhance its genotoxicity by suppressing NER capacity.2022-09-01T00:00:00ZAssessing Groundwater Dynamics and Potentiality in the Lower Ganga Plain, India
http://scholars.ntou.edu.tw/handle/123456789/22107
標題: Assessing Groundwater Dynamics and Potentiality in the Lower Ganga Plain, India
作者: Mondal, Biraj Kanti; Sahoo, Satiprasad; Das, Rima; Mishra, Prabuddh Kumar; Abdelrahman, Kamal; Acharya, Aditi; Lee, Ming-An; Tiwari, Anuj; Fnais, Mohammed S.
摘要: The present study intended to assess groundwater storage dynamics (GWS) and identify potential zones using the Multi-Criteria Decision Making (MCDM) method and geospatial technology in the Murshidabad district of West Bengal, India. The study district is located in the Ganga-Padma-Bhagirathi rivers' floodplain and covers approximately a 5324 km(2) area, comprising 26 blocks in five sub-divisions. The study portrayed a quantitative investigation of the pre-monsoon and post-monsoon season's variability of GWS from 2000 to 2020, taking Landsat TM/Landsat 8 OLI/SRTM satellite data. The geo-spatio-temporal analysis of groundwater storage variability for 20 years was carried out by such remotely sensed data with the geospatial method to portray the dynamics and uncover the potential zones of GWS using various cartographic and statistical techniques. We determined nine parameters for the study, and the analytical hierarchy process (AHP) method was employed for the computation. The present estimation and assessment include the MCDM method, covering assorted parameters and the variations and aspects of GWS in the pre- and post-monsoon seasons from 2000 to 2020. The outcome illustrates that a decline in water storage has taken place in most of the blocks of Murshidabad district on average during the study period, which indicates a water stress provison in the near future. However, the micro (block)-level scenario of the spatiotemporal dynamics of GWS and the potential zonation in the Murshidabad District were investigated to form a location-specific micro-level arrangement for the sustainable management of water.2022-07-01T00:00:00ZInvestigation and Estimation of Groundwater Level Fluctuation Potential: A Case Study in the Pei-Kang River Basin and Chou-Shui River Basin of the Taiwan Mountainous Region
http://scholars.ntou.edu.tw/handle/123456789/22106
標題: Investigation and Estimation of Groundwater Level Fluctuation Potential: A Case Study in the Pei-Kang River Basin and Chou-Shui River Basin of the Taiwan Mountainous Region
作者: Chen, Nai-Chin; Wen, Hui-Yu; Li, Feng-Mei; Hsu, Shih-Meng; Ke, Chien-Chung; Lin, Yen-Tsu; Huang, Chi-Chao
摘要: Featured Application Investigating the spatial and temporal dynamic behavior of groundwater level fluctuation in mountainous areas using the proposed approach can assist in obtaining effective strategies for developing and managing groundwater resources. The analysis of the spatiotemporal characteristics of groundwater level variation is a prerequisite for evaluating groundwater potential or underpinning aquifer sustainability development in hydrogeological engineering practices. This study explores the dominant influencing factors that control groundwater dynamics and develops an estimation of groundwater level fluctuation (GWLF) potential in the complex aquifer systems of mountainous areas. Eight natural environmental factors, including slope, drainage density, land use, lithology, hydraulic conductivity, porosity, groundwater depth, and regolith thickness, have been selected as influencing factors, and the feature scores for different factors associated with GWLF potential were given with the expert scoring method. The weighting coefficients of individual influencing factors for wet/dry seasons were determined using the pseudo-inverse method based on the groundwater level data of 18 well stations observed from 1 November 2011 to 31 October 2019 in Taiwan mountainous areas. The results show that the weighting coefficients of these factors in controlling GWLF potential are variable and affected by seasonal and annual rainfall conditions. Based on the determined weighting coefficients, the spatial distribution of GWLF potential can be effectively produced. Finally, the simulated GWLF potential results were verified by comparing the observed data. The verification result demonstrates that the developed model can predict the spatial GWLF distribution based on the groundwater level data from a few wells.2022-07-01T00:00:00ZEffects of dietary Lactobacillus reuteri and Pediococcus acidilactici on the cultured water qualities, the growth and non-specific immune responses of Penaeus vannamei
http://scholars.ntou.edu.tw/handle/123456789/22108
標題: Effects of dietary Lactobacillus reuteri and Pediococcus acidilactici on the cultured water qualities, the growth and non-specific immune responses of Penaeus vannamei
作者: Wu, Yu-Sheng; Chu, Yu-Ting; Chen, Yin-Yu; Chang, Chao-Sheng; Lee, Bao-Hong; Nan, Fan-Hua
摘要: This study discussed the effects of two types of lactic acid bacteria, Lactobacillus reuteri (L. reuteri) and Pediococcus acidilactici (P. acidilactici), on the growth and nonspecific immunity of Penaeus vannamei (P. vannamei) and developed probiotic applications for shrimp cultivation. This study incorporated two types of lactic acid bacteria in shrimp feed through spraying. The shrimps were grouped according to the type and concentration of effective bacteria incorporated into their feed. This research was separated into 3 individual feeding treatment as control, L. reuteri (Lr groups) and P. acidilactici (Pa groups). The shrimp was feeding with 10(3), 10(5), and 10(7) cfu/feed (g) L. reuteri namely as Lr3, Lr5, and Lr7. The shrimp was feeding with 10(3), 10(5), and 10(7) cfu/feed (g) P. acidilactici were named Pa3, Pa5, and Pa7, respectively. Through 8 weeks of feeding, the results revealed that the use of shrimp feed incorporated with lactic acid bacteria did not cause negative effects on water quality. The testing items include ammonia-nitrogen concentration, nitritenitrogen concentration, and total vibrio count in the water. In addition, the lactic acid bacteria concentration in the water were in the range of 1.33 +/- 0.58 x 10(1) to 9.77 +/- 1.34 x 10(2) cfu/mL. Observations of the growth performance of the white shrimps after 8 weeks of feeding revealed that both bacteria were beneficial to shrimp growth. In particular, group Lr7 had the highest percentage weight gain (107.99 +/- 3.92%), special growth rate (1.93 +/- 0.07%), feed conversion ratio (3.34 +/- 0.05), and survival rate (97.22 +/- 4.81%). Furthermore, observations of the nonspecific immunity reactions of the white shrimps after 4 weeks of feeding indicated that on day 4, the total number of haemocyte in shrimps in groups Lr5, Lr7, Pa3, and Pa5 significantly increased. On days 1 and 4, the phenoloxidase activity and superoxide axion production rates of the Lr group and Ls group increased. This phenomenon was the most significant in group Lr7, and the effect continued until day 28. After day 7, the phagocytic rate of groups Lr5 and Lr7 significantly increased. In addition, Lr and Pa groups exhibited significant increases in the phagocytic index after days 4 and 14, respectively. This phenomenon was also the most significant in group Lr7.2022-08-01T00:00:00Z