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Adjuvant Molecular Hydrogen Facilitates Immunosuppressant Tapering and Sustained Remission in Systemic Lupus Erythematosus: A Case Report

Thu, 01 Jan 2026 00:00:00 GMT

標題: Adjuvant Molecular Hydrogen Facilitates Immunosuppressant Tapering and Sustained Remission in Systemic Lupus Erythematosus: A Case Report 作者: Lin, Hong-Jie; Lu, Jeng-Wei; Ho, Yi-Jung; Lui, Shan-Wen; Hsieh, Ting-Yu; Wang, Kuang-Yih; Liu, Feng-Cheng 摘要: Background/Aim: Management of systemic lupus erythematosus (SLE) remains challenging: intensive immunosuppression increases infection risk, whereas dose reduction may trigger disease flares. Molecular hydrogen (H2), a selective antioxidant with anti-inflammatory properties, may serve as an adjunct therapy, potentially maintaining disease control while reducing Case Report: We present a 43-year-old female with longstanding SLE who experienced recurrent hospitalized infections (pneumonia and complex urinary tract infections) under high-dose immunosuppression in 2022. A subsequent attempt to reduce her medication dosage provoked a significant disease flare in January 2023, with anti-double stranded DNA (anti-dsDNA) levels surging to 890 IU/ml and complement depletion. Oral H2 capsule therapy was initiated as an adjunct. The patient exhibited rapid and robust serologic improvement, allowing for the complete discontinuation of mycophenolic acid within one month. Over an extended follow-up through early 2026, her regimen was successfully de-escalated to a minimized monotherapy of prednisolone 10 mg/day. Her anti-dsDNA levels stabilized within a strictly normal range (<15 IU/ml), with normalized complement levels, no further infections, and profoundly reduced fatigue. Flow-cytometric profiling revealed a coordinated reprogramming of adaptive immunity toward a controlled, non-senescent state, including a homeostatic reset in the B-cell compartment and decreased active-disease-associated T cells. Conclusion: Adjuvant molecular hydrogen therapy stabilized active SLE, enabling substantial reduction of conventional immunosuppressants while maintaining durable clinical and serologic remission. This strategy may represent a promising immunosuppressant-sparing approach and warrants validation in controlled trials.

Enhanced therapeutic effect of chemotherapy with magnetic mesoporous silica nanoparticle-mediated synergistic hyperthermia therapy

Thu, 01 Jan 2026 00:00:00 GMT

標題: Enhanced therapeutic effect of chemotherapy with magnetic mesoporous silica nanoparticle-mediated synergistic hyperthermia therapy 作者: Lee, Cheng-Chang; Lin, Yuan-Ting; Huang, Kai-Qun; Hwang, Jih-Shang; Lin, Hsiu-Mei 摘要: This research combined chemotherapy and magnetic hyperthermia therapy in a nano-drug delivery system based on mesoporous silica. The material exhibited dual imaging ability by adding Eu3+ ions to the silica framework with an iron oxide core, including IVIS and T2 MRI. By further grafting temperature-sensitive polymers acting as gatekeepers, the chemo drug camptothecin (CPT) was caged inside the pore until the temperature increased to 42 degrees C due to the magnetic hyperthermia effect. Moreover, tumor cells overexpress the folic acid (FA) receptor due to the need for FA molecules during their growth. It is promising that by grafting FA onto nanoparticles, tumor cells take up more material, which increases the specificity of the nanoparticles. MMSN-Eu-PEGMA-FA@CPT was successfully taken up by A549 cells, as observed with confocal laser scanning microscopy (CLSM), and exhibited great inhibition of cancer cell A549 growth. At a concentration of 25 & micro;g mL-1 under AMF, the cell viability of A549 cells was 56.2%. This is due to the synergistic effect of chemotherapy and hyperthermia therapy.

Cocktail-like alginate-derived carbonized nanogels with diverse moieties attenuate tumor metastasis via potential multi-pathway modulation

Thu, 01 Jan 2026 00:00:00 GMT

標題: Cocktail-like alginate-derived carbonized nanogels with diverse moieties attenuate tumor metastasis via potential multi-pathway modulation 作者: Wang, Chen-Yow; Mao, Ju-Yi; Unnikrishnan, Binesh; Chan, Kai-Min; Sarkar, Saumyadip; Lin, Lin; Hsu, Pang-Hung; Huang, Yu-Fen; Harroun, Scott G.; Huang, Chih-Ching; Chen, Shiow-Yi 摘要: Carbonized nanomaterials possess diverse functional moieties that complicate biomedical use but also confer multi-target therapeutic potential. Here, carbonized nanogels (CNGs) were synthesized from sodium alginate (Alg) via mild pyrolysis, yielding ultrasmall graphene-like domains embedded in a crosslinked nanogel matrix enriched with bioactive moieties. These features endowed Alg-CNGs with antioxidative, anti-inflammatory, and membrane-perturbing properties, enabling modulation of cellular signaling relevant to cancer progression. In vitro, Alg-CNGs suppressed epithelial-mesenchymal transition in 4T1 triple-negative breast cancer cells by increasing E-cadherin and decreasing N-cadherin and vimentin, thereby attenuating EMT-associated motility as evidenced by reduced migration/invasion and marked perturbation of actin organization and adhesion-related dynamics. Quantitative proteomics coupled with Ingenuity Pathway Analysis further indicated attenuation of metastasis-associated networks, including RhoA/Rac1/Cdc42-integrin signaling and PI3K/AKT, PTEN, and ERK/ MAPK pathways. In an orthotopic breast cancer model, Alg-CNGs accumulated selectively in tumors and reduced pulmonary metastasis by 90% without systemic toxicity. Structure-function analysis indicated that phenolic, lactone, and quinone moieties on the CNGs may contribute to regulating oxidative stress, actin remodeling, and receptor-mediated signaling. These findings demonstrate that Alg-CNGs exert cocktail-like" multifunctional actions

Molecular Hydrogen Reverses Sepsis-induced Immunoparalysis: Insights from Longitudinal Deep Immunophenotyping

Thu, 01 Jan 2026 00:00:00 GMT

標題: Molecular Hydrogen Reverses Sepsis-induced Immunoparalysis: Insights from Longitudinal Deep Immunophenotyping 作者: Wu, Chang-Lung; Lu, Jeng-Wei; Ho, Yi-Jung; Lui, Shan-Wen; Hsieh, Ting-Yu; Wang, Kuang-Yih; Liu, Feng-Cheng 摘要: Background/Aim: Refractory septic shock and post-cardiac arrest syndrome (PCAS) can induce lethal immunoparalysis. However, longitudinal evidence on the effects of molecular hydrogen on deep human immunophenotyping remains scarce. Case Report: We report the case of a 49-year-old man with severe tricuspid regurgitation and Child-Pugh class B alcoholic cirrhosis who developed severe pneumonia, refractory septic shock, and PCAS after high-energy thoracic trauma. At a therapeutic impasse marked by high levels of N-terminal pro-B-type natriuretic peptide (NT-proBNP) (>35,000 pg/ml) and profound immune depletion, adjuvant hydrogen inhalation was initiated, which led to a 64% reduction in myocardial stress and temporary clinical stabilization. Deep longitudinal flow cytometry unveiled system-wide immunological reprogramming. In the T-cell compartment, hydrogen induced a biphasic resolution of exhaustion in T-helper cells marked by an immediate decline in expression of FAS cell surface death receptor (FAS; also known as CD95) and asynchronous normalization of expression of programmed cell death protein 1 (PD1) and T-cell immunoglobulin and mucin domain 3 (TIM3), alongside bidirectional restoration of physiological immune checkpoints in cytotoxic T-cells. Furthermore, we observed a sequential, compensatory recovery of the regulatory T-cell (Treg)/type 1 regulatory T-cell (Tr1) immune-braking system. In the humoral compartment, despite prolonged bone marrow suppression and loss of transitional/regulatory B-cells, hydrogen therapy was associated with a V-shaped recovery of plasma and double-negative (for both cluster of differentiation 27 and immunoglobulin D) B-cells. It also appeared to protect switched memory B-cells by markedly reducing FAS expression, suggesting an anti-apoptotic effect. Conclusion: Although the patient ultimately succumbed to irreversible chronic comorbidities on day 86, this case of severe tricuspid regurgitation and Child-Pugh class B alcoholic cirrhosis demonstrates profound yet transient immunological rescue. By disrupting the cycle of immune exhaustion and exerting anti-apoptotic effects, molecular hydrogen may represent a promising immunomodulatory adjunct in severe sepsis.