Cryopreservation of the grouper, Epinephelus malabaricus, sperm. In：A. P. Scott, J.P. Sumpter, D.E. Kime and M. S.Rolfe (eds.)

Abstract

Suspension solution of sperm from sliced testes or suction of sperm from genital papilla with capillary tubes were found to be efficient means to obtain raw milt. DMSO and glycerol with 15% INRA Menezo B2 medium gave good cryoprotective functions. The potential of microwave thawing was also shown. Hamilton-Thorn Motility analyzer was used to compare fresh and long-term cryopreserved sperm. Artificial propagation using 90 min-, 1 day-, 17 day-, 54 day- and 291 day-cryopreserved sperm with fresh eggs resulted in fertilities of 95.7% (vs. 93.8% in control), 93.2% (vs. 65.6%), 85.0% (vs. 65.6%), 93.3% (vs. 91.9%) and 84.6% (vs. 91.9%), respectively.