Application of honey in cryopreservation of sperm of milkfish (Chanos chanos) and black porgy (Acanthopagrus schlegeli). In：D.R. Idler, L.W. Crim and J.M. Walsh (eds.)

Abstract

For cryopreserving sperm of marine fin-fish, extenders are needed to dilute viscid milt and to provide nutrients for long-term storage. The aim is to find a more functional, convenient and inexpensive extender for milkfish and black porgy sperm preservation. Pure honey is not only a natural bacteria inhibitor but also reasonably priced and readily available. The result of this study shows that 1% honey (449 mOsm/kg) was an optimum substitute for milkfish serum in the preserving of milkfish sperm in terms of prefreezing and post-thawing motility. Nevertheless, fertility needs to be studied in the near future. On the other hand, 0.5% honey (210 mOsm/kg) was used to substitute 5.6% glucose in preserving black porgy sperm and rather satisfactory prefreezing, frozen-thawed, and three-day frozen-thawed motility as well as fertility were obtained.