Gonadotropin induced synchronous changes of morphology and gonadol development in the Japanese eel Anguilla japonica

Abstract

In a previous study, we had observed synchronous changes of morphology and gonadal development in wild Japanese eel during the silvering process. In this study, we aimed to clarify if gonadotropin is the key hormone responsible for this phenomenon. Yellow eels captured in the Kaoping River were repeatedly injected with human chorionic gonadotropin (HCG), and changes of morphology and gonadal development were examined. After five weekly injection of HCG at a dosage of 0.75 IU/g body weight, eels was classified into well-responsive and poor-responsive groups according to skin coloration. Approximately 50 % of males and 20 % of females were responsive to become silver eels. Mean age, total length, body weight, condition factor, gonadosomatic index (GSI), fin-index (FI), hepatosomatic index (HSI) and ocular index (OI) were significantly higher in well-responsive eels than in poor-responsive eels. In contrast, the mean digestosomatic index (DSI) significantly decreased in well-responsive eels. OI was positively correlated and DSI was negatively correlated with the GSI in both sexes. These results indicated that well developed eels are more sensitive to HCG treatment, and the skin color, eye size, gonadal development and digestive tract shrinkage were synchronous after HCG injection. The pituitary — gonad axis plays important role on eel silvering.