http://scholars.ntou.edu.tw/handle/123456789/14479
Title: | Salinity preference of silvering Japanese eel Anguilla japonica:evidence from pituitary prolactin mRNA levels and otolith Sr:Ca ratios | Authors: | Han, Y.S. Yu, Y.L. Tzeng, W.N. I-Chiu Liao |
Issue Date: | Sep-2003 | Publisher: | Marine Ecology Progress Series | Journal Volume: | 259 | Start page/Pages: | pp.253-261 | Abstract: | The salinity preference of Japanese eel Anguilla japonica during silvering was validated from the viewpoints of physiological ecology by examining the pituitary prolactin (PRL) mRNA expression and the otolith strontium: calcium (Sr:Ca) ratios of the eels of different sexual maturity stages collected in an estuary of Taiwan. The PRL possessed a putative signal peptide of 24 amino acids and a mature peptide of 185 amino acids. RT-PCR and real-time quantitative PCR analyses indicated that the transcript levels of PRL mRNA significantly decreased from yellow to silver stage. On the contrary, the Sr:Ca ratios at the otolith edge of silver eels, which recorded their latest experience of salinity history, significantly increased. The increase in otolith Sr:Ca ratios and the decrease in PRL mRNA expression are consistent with the timing of migration from freshwater to seawater during silvering of the eel. |
URI: | http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45063 http://scholars.ntou.edu.tw/handle/123456789/14479 |
DOI: | 10.3354/meps259253 |
Appears in Collections: | 海洋中心 |
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