Purification and Characterization of Fractions Containing Polysaccharides from Talinum triangulare and Their Immunomodulatory Effects

Abstract

Previous studies identified that extracts of Talinum triangulare rich in flavonoids and phenolic acids showed antioxidative and immunomodulatory activities. In this study, the L9 orthogonal array was used to determine the optimal extraction conditions for water-extracted polysaccharides of T. triangulare (TTP) by hot reflux extraction and ultrasonic assisted extraction (UAE) methods. Results showed that while both extraction methods obtained a maximum polysaccharide yield of 3.1%, the optimal conditions for obtaining TTP was by UAE method. TTP was separated into large (LTTP) and small (STTP) molecular weights by dialysis. Since LTTP showed better effects than STTP in inducing macrophages to produce nitric oxide (NO) and indirectly inhibiting human cervical cancer HeLa cells, six different LTTP fractions were separated using anion-exchange chromatography. Contents of polysaccharides, triterpenoids, polyphenols, and proteins and molecular weights of major polysaccharide in each fraction were analyzed. The F1 fraction of LTTP, which showed the highest inducing ability of mouse RAW264.7 macrophages to secrete NO and tumor necrosis factor-alpha, showed the most significant indirect inhibitory effect of human colon cancer SW620 cells. These results suggest that LTTP, especially the F1 fraction, of T. triangulare may be used in health foods or Chinese medicine for its immunomodulatory potential.