ANTIOXIDANT CAPACITY AND METABOLIC RESPONSE OF RED STRIPED SNAPPER Lutjanus erythropterus FED DIET WITH ASTAXANTHIN AND/OR OXIDIZED OIL

Abstract

This study evaluated the effects of astaxanthin (AX) on antioxidant capacity, metabolic response, and liver AX concentration of red striped snapper (Lutjanus erythropterus) (20.05 +/- 1.65 g) fed with a diet with or without high dosage of oxidized oil (OX). The commercial diet served as control (C). Fish were fed with a diet containing 240 mg/kg AX, 100 ml/kg OX, or combination of 240 mg/kg AX and 100 ml/kg OX (AX + OX) for a day. Antioxidant capacity (superoxide dismutase, glutathione peroxidase, and glutathione reductase) and metabolic response (glucose, triglycerides, and lactate) were monitored at 0, 6, 12, 24, 48 and 72 h while liver AX concentration was analyzed at 72 h after feeding. AX- and AX + OX-fish had more improved antioxidant capacity and metabolic response than C- and OX-fish at 6 and 12 h. Moreover, OX-fish had higher glutathione reductase, glucose, triglycerides, and lactate concentrations than AX-fish at 6 and 12 h. Treatments that received AX showed higher liver AX concentration as expected. Overall, this study demonstrated that supplementation of AX in the diet significantly stabilizes both antioxidant capacity and metabolic responses of L. erythropterus under a prooxidant circumstance associated with oxidized fish oil intake.