http://scholars.ntou.edu.tw/handle/123456789/20804
標題: | Nanoplasmonic biochips for rapid label-free detection of imidacloprid pesticides with a smartphone | 作者: | Lee, Kuang-Li You, Meng-Lin Tsai, Chia-Hsin Lin, En-Hung Hsieh, Shu-Yi Ho, Ming-Hsun Hsu, Ju-Chun Wei, Pei-Kuen |
關鍵字: | LINKED-IMMUNOSORBENT-ASSAY;NEONICOTINOID INSECTICIDES;FANO RESONANCES;ARRAYS;TRANSMISSION;IMMUNOASSAY;BIOSENSORS;SENSORS;RISKS | 公開日期: | 15-一月-2016 | 出版社: | ELSEVIER ADVANCED TECHNOLOGY | 卷: | 75 | 起(迄)頁: | 88-95 | 來源出版物: | BIOSENS BIOELECTRON | 摘要: | The widespread and intensive use of neonicotinoid insecticides induces negative cascading effects on ecosystems. It is desirable to develop a portable sensitive sensing platform for on-site screening of high-risk pesticides. We combined an indirect competitive immunoassay, highly sensitive surface plasmon resonance (SPR) biochip and a simple portable imaging setup for label-free detection of imidacloprid pesticides. The SPR biochip consists of several capped nanoslit arrays with different periods which form a spectral image on the chip. The qualitative and semiquantitative analyses of pesticides can be directly observed from the spot shift on the chip. The precise semiquantitative analyses can be further completed by using image processing in a smartphone. We demonstrate simultaneous detection of four different concentrations of imidacloprid pesticides. The visual detection limit is about 1 ppb, which is well below the maximum residue concentration permitted by law (20 ppb). Compared to the one-step strip assay, the proposed chip is capable of performing semiquantitative analyses and multiple detection. Compared to the enzyme-linked immunosorbent assay, our method is label-free and requires simple washing steps and short reaction time. In addition, the label-free chip has a comparable sensitivity but wider working range than those labeling techniques. (C) 2015 Elsevier B.V. All rights reserved. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/20804 | ISSN: | 0956-5663 | DOI: | 10.1016/j.bios.2015.08.010 |
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