The portable fluorescence detection system matched with PDMS microfluidic biochip for DNA hybridization detection

Abstract

The self-made fluorescence detection system was integrated to detect the biological signal of DNA sample. And the portable fluorescence detection system with four channels microfluidic biochip was finished for DNA hybridization.

In the biochip preparation, the PDMS cast-forming process was used to fabricate microfluidic biochip with width 150 μm and height 200 μm. In the fluorescence detection system, four sets of independent 532 nm green lasers were adopted to illuminate the four detection areas of the microfluidic biochip for fluorescence sample excitation, then the voltage of excitation fluorescence signal was obtained. Then the PSOC embedded system was utilized synchronously to display the four voltage values on LCD, in the meantime, the voltage value was transferred to the computer for recording.

From the experimental result of DNA hybridization, it can be seen that portable fluorescence detection system can be utilized to measure four fluorescence signals of DNA samples at the same time. Moreover, due to the hybridization specificity of fluorescence probe ET996-Hex, the DNA hybridization of Edwardsiella tarda BCRC 16711 and Edwardsiella tarda BCRC 16702 can be judged successfully for fluorescence probe ET996-Hex. The portable fluorescence detection system had the capability to distinguish disease source.