Alteration of Phenotype in Ornamental Fish by Genetic Engineering Technique

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Project title

Code/計畫編號

90農科-2.1.1-漁-F1(6)

Translated Name/計畫中文名

以基因工程改良觀賞魚表現型

Project Coordinator/計畫主持人

Jenn-Kan Lu

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1046509

Year

2001

Start date/計畫起

01-02-2001

Expected Completion/計畫迄

31-12-2001

Bugetid/研究經費

650千元

ResearchField/研究領域

生物技術(農)
漁業

( 一 )全程計畫目標: 本計畫利用遺傳工程改變控制色素基因表現或以轉殖螢光基因, 改變轉基因魚的體色( 基因表現型 ), 增加觀賞魚的價值與品質的提昇, 增加我國觀賞魚在國際市場的競爭力.( 二 )本年度計畫目標: 本計畫的長程目標是以基因工程改良觀賞魚表現型( 包括: 顏色、體型、外觀 )作為商業之用.短程目標是以基因轉殖技術或其他遺傳工程技術生產不同體色之觀賞魚.為達到上述目標, 本年度我們將設計下列實驗: 1.建立觀賞魚基因轉殖技術( 包括: 電破法、直接生殖腺微脂體基因轉殖法、顯微注射法 ); 2.使用不同具有組織專一性( 表皮細胞、肌肉 )調控表現的啟動子( promoters )黏合不同顏色( 紅、綠、藍色等 )螢光蛋白( fluorecent protein ), 3.使用可抑制色素形成之tyrosinase基因表現的分生技術( 包括antisense( 反意 )RNA, RNAi技術 )來改變觀賞魚體色產生新品種, 4.利用基因治療法( gene therapy )改變觀賞魚外觀( 例如: 脂肪瘤基因移植 ), 5.生產體型較大品種觀賞魚.待上述問題解決後, 獨特體色、外觀的轉基因觀賞魚種將可商品化.( 三 )本年度預期效益及其評估指標: 利用基因轉殖、分子生物、基因工程與育種技術生產改良觀賞魚( 包括: 顏色, 體型 )其效益是多層的: ( 一 )建立獨特品系, 使我國觀賞魚產業更具國際競爭力, 增加觀賞魚附加價值, 提高利潤, ( 二 )增加對顏色基因表現之調控機制在低等脊椎動物之了解, 進而操控基因表現.欲發展轉基因觀賞魚技術首先要決定, a )欲基因修改之觀賞魚品種, b )各種改變所需之管理方式, c )發展成本與其他替代遺傳工程技術, d )可否做多重基因修改, e )消費者的反應-市場效應, f )整個產業或單一生產業者有關, g )基因庫/育種登錄.至今, 生產轉基因魚、蝦、貝類常規方法, 但是為求了解以現代基因工程產生外觀顏色改變之轉基因觀賞魚其商業價值及其商業化之可行性, 仍需要一系列的突破.本計畫評估指標包括: ( 一 )1.成功生產轉基因觀賞魚( 金魚、斑馬魚、血鸚鵡 )2.提高基因轉殖生產觀賞魚之效率, ( 二 )1.尋求適當之啟動子序列( promoter )來調控外來螢光基因或體色素轉基因之表現( expression )2.決定轉基因觀賞魚可使其最佳表現之生理, 營養與環境因素.3.優良品系之建立.上述問題解決後, 獨特體色、外觀的轉基因觀賞魚種將可商品化. After photography, keeping ornamental fish is the second biggest hobby in the US.It is estimated that the global trade is worth US$1.6billions annually.Due to the environmental lobby against the harvest of wild fish and the increasing problems of disease and water qualities experienced by ornamental fish farmer in developing countries.An opportunity exists at Taiwan to develop its ornamental sector.The major markets for ornamental fish are the Far East, the United States of America and the European Union ( EU ).Although the Far East is the principal source of both supply and demand for ornamental species, there is considerable movement of fish between these three areas.( 1 )Long term goals: The long term goal of this project is to alter the phenotype of valuable ornamental fish ( such as coloration, emission various fluorescent light, various phenotypical changes )by employing modern genetic engineering technique.Desirable strains ( e.g.coloration, emission of various fluorescent light, larger size, various phenotypical changes )would help Taiwan ornamental fish producers compete with foreign sources.( 2 )This year short term goals: To fulfill this long term goal, several short term goal will proposed as following: 1 )establish gene transfer technique in ornamental fish; 2 )select proper gene construct for various phenotypical altered transgenic fish; 3 )isolation and characterization genes which regulate pigmentation in fish.( such as inhibitory of tyrosinase gene ); 4 )select proper promoter which driven transgene expression.( 3 )Expected results and evaluation indicators: The goal of this program is to produce phenotypical altered transgenic ornamental fish, an achievement would have significant impact on ornamental fish grower in the Taiwan.While these technological efforts lie outside the research topics addressed in the application, their prospects for success will enhanced by the research proposed here.1 )Establish transgenic ornamental fishe ( gold fish, zebrafish, red parrot )2 )Improve success rate of production of transgenic ornamental fish 3 )Employment and searching proper promoter sequence for regulating transgene expression 4 )Optimize culturing conditions for transgene expression 5 )Establish transgenic ornamental fish lines ( strains ).

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Alteration of Phenotype in Ornamental Fish by Genetic Engineering Technique

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