Self-Powered Wireless Biosensor System for Real-Time Monitoring of Blood Glucose/Cholesterol Levels in Grouper Fish

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Project title

Code/計畫編號

MOST103-2633-E019-001

Translated Name/計畫中文名

可自體產生電力之植入式石斑魚生理狀態無線感測裝置之開發

Project Coordinator/計畫主持人

Shih-Hao Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=8392147

Year

2014

Start date/計畫起

01-10-2014

Expected Completion/計畫迄

01-09-2015

Bugetid/研究經費

418千元

ResearchField/研究領域

醫學工程

"石斑魚是台灣重要內外銷經濟魚種，近年來受到魚類病毒感染和寒害的影響，石斑養殖成功率因而降低，因此良好的健康管理不僅可以避免疾病的發生，縱使爆發疫情，也能夠及早發現並採取防疫措施，所以若能即時監控石斑魚魚體的生理狀態，進行定期生理檢測與異常警示，一旦偵測到異常狀態，可即時進行緊急預防處置，可有效減少養殖業者因魚類疾病與寒害所造成的損失。由於體內血液中膽固醇與血糖的驟降變化是生理狀態異常與抗細菌/病毒感染力降低的一個有用的指標，在養殖漁場中即時監測活體魚體內血糖和膽固醇的變化可用來確定魚體的生理健康的狀態，進行定期生理檢測與異常警示，因此本計畫將擬以二年期以結合酵素型生物感測器與酵素型燃料電池進行可自體產生電力之植入式石斑魚生理狀態無線感測裝置的開發，預計開發出探針型酵素電極感測器並連接到無線訊號傳輸裝置，藉以即時感測魚體內血糖/膽固醇的變化，此探針型酵素電極感測器將以人為方式由外部植入魚眼球後方鞏膜內，可接觸眼球鞏膜組織間液(eyeball interstitial sclera fluid, EISF) 進行血糖/膽固醇的量測，眼球鞏膜組織間液(EISF)為透明組織液，不會造成血液凝固或球蛋白凝結於感測器探針上造成電流訊號干擾而無法長期量測。為能提供電極感測器恆電位儀與無線訊號傳輸裝置所需電力，同時也將開發探針式葡萄糖氧化酵素燃料電池，而此探針將由外部植入魚眼球後方鞏膜內，接觸眼球鞏膜組織間液，催化葡萄糖產生電力，作為探針型酵素電極感測器與無線傳輸裝置的電力來源。所謂「預防勝於治療」，本計畫提出的生理狀態無線感測裝置可進行定期生理檢測與異常警示，可有效減少養殖業者因魚類疾病與寒害所造成的巨大損失。" "Periodic checks of fish health and the rapid detection of abnormalities are thus necessary at fish farms. Blood glucose or plasma total cholesterol levels are significantly related to fish mortality following bacterial challenge, and are thus a good indicator of the general health of fish. In this project, we plan to develop a wireless enzyme sensor system integrated with an enzymatic biofuel cell as energy sources to determine blood glucose or cholesterol levels in fish (Taiwan Grouper). The wireless enzyme biosensor was constructed with Pt-Ir wire as the working electrode and Ag/AgCl paste as the reference electrode. To avoid blood coagulation and proteins coalescing on the sensor, we plan to implant the sensor in the fluid under the scleral surface of the eyeball (EISF). The EISF is presumed to reflect the levels of most blood components and does not include the substances contained in blood that inhibit sensor measurement. To provide the electrical power for wireless enzyme sensor, we plan to develop and integrate an enzymatic biofuel cell into the wireless enzyme sensor. We plan to fabricate a miniature assembly that consists of a needle bioanode for accessing biofuels in EISF and a gas-diffusion biocathode for utilizing the abundant oxygen in water. The needle anode is inserted into fish eye ball to contact with the EISF to produce electrical power. We will setup a wireless monitoring system to achieve a rapid and convenient method for real-time monitoring of blood glucose or cholesterol levels in free-swimming fish."

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Self-Powered Wireless Biosensor System for Real-Time Monitoring of Blood Glucose/Cholesterol Levels in Grouper Fish

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