Development on the production technology of microtube array membrane-immobilized butanolgenic cell for biobutanol from Sargassum sp. and its life cycle assessment

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Project title

Code/計畫編號

MOST105-ET-E019-001-ET

Translated Name/計畫中文名

以微管束陣列膜固定產丁醇菌發酵生產馬尾藻生質丁醇之技術研發與製程之生命週期評估

Project Coordinator/計畫主持人

Chorng-Liang Pan

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

林泓廷

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=11711610

Year

2016

Start date/計畫起

01-01-2016

Expected Completion/計畫迄

01-12-2016

Co-Investigator(s)

Hong-Ting Lin

Bugetid/研究經費

958千元

ResearchField/研究領域

能源工程

"本研究計畫之目的為研發自褐藻馬尾藻(Sargassum sp.) 生產生質丁醇的發酵技術。主要工作包括將馬尾藻或馬尾藻熱萃多醣以3-5 組多醣分解酵素降解為可供產丁醇梭孢桿菌代謝利用之單醣產物，同時探討數項關鍵反應條件如水解液之溫度與pH 值等對於可發酵糖產率的影響，並用HPLC協助確認水解液中寡醣與單醣之組成與含量。馬尾藻多醣水解液即利用耐鹽(ST) 耐丁醇(BT) 產丁醇菌衍生株以批式與饋料批式發酵技術探討發酵中之重要影響因子，如發酵液中之可發酵醣類之組成與濃度、發酵溫度、pH 值、種入之產丁醇菌株菌量與菌齡、鹽濃度、以及重要中間產物丁酸等之變化。並擬利用微管束陣列膜(Microtube array membrane, MTAM) 固定化菌酛細胞方法以及氣提 (Gas stripping) 回收技術進一步提升馬尾藻丁醇的發酵產率。以及瞭解到達發酵終點時發酵液中之醣類與醇類等之組成與含量，做為未來持續提升自各類海藻生產生質丁醇的研發基礎。並以生命週期評估(LCA) 的方法瞭解產製龍鬚菜生質丁醇之生產成本與所產生之環境效益(對降低碳足跡之正面影響)。本年度研究計畫期望能達成自褐藻馬尾藻經酵素水解與發酵產出10-12%生質丁醇的研發目標。" "The objective of this study is to develop potential fermentation techniques for producing bio-butanol from brown alga Sargassum sp. Sargassum sp. and its hot water extracted polysaccharides (PS) are target Experimental Materials (EM) for the following enzymatic digestions. Three to five variables of PS digesting enzyme mixtures degrade EM to obtain fermentable simple sugars (FSS), which can be further metabolized and fermented to butanol by clostridia. Several key factors for optimal FSS yield such as digesting temperature and pH value are evaluated. Amount and composition of oligosaccharides and monosaccharides from Sargassum hydrolysates are analyzed by HPLC. Utilizing batch type and fed batch type of fermentation, hydrolysate of Sargassum sp. is fermented by butanol producing clostridia, and the critical production factors are assessed on such as yield of FSS, fermentation temperature, pH value, amount and age of starters, salt concentration, and the change of butyric acid. In addition, microtube array membrane (MTAM) immobilized cell and gas striping techniques are applied to promote the fermentation yield of bio-butanol, and composition and concentration of carbohydrates and alcohols from fermented substance in the end of reaction are expected as meaningful research foundation for future developing bio-butanol production from various algae species. The changes of cost and carbon footprint of different bio-butanol production procedure from Sargassum sp. are evaluated by Life Cycle Assessment (LCA) method, and these results could be used to lower down both production cost and CO2 releasing amount. The related environmental benefit of the production of Sargassum bio-butanol is also being estimated. The study goal of this project is to produce 10-12 g butanol/100 g of Sargassum sp. via hydrolysis and fermentation process."

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Development on the production technology of microtube array membrane-immobilized butanolgenic cell for biobutanol from Sargassum sp. and its life cycle assessment

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