Plankton Ecological Processes in Subtropical-Shelf Ecosystem: Metabolism Activity and Mixotrophic Mechanism of Picoeukaryotes(III)

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基本資料

Project title

Code/計畫編號

MOST107-2611-M019-002-MY3

Translated Name/計畫中文名

副熱帶陸棚浮游生物食物網生態過程研究-總計畫及子計畫：副熱帶陸棚浮游生物食物網生態過程研究: 超微真核藻類生理活性與混營行為機制(III)

Project Coordinator/計畫主持人

Kuo-Ping Chiang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=13320317

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

2215千元

ResearchField/研究領域

海洋科學

超微真核藻類在大洋中高生物量且高多樣性，是海洋生態系中重要的基礎生產者。因此了解超微真核藻類在大洋中的成長有助於了解其的生態分布與碳循環。核糖體RNA的含量常被用來當作細胞成長與活性的指標，但是此應用卻很少用在超微真核藻類。18S rRNA/rDNA的量測方法是利用添加已知濃度外來的的RNA/DNA標準品。之後利用定量聚合酶連鎖反應來計算18S 與標準品在 DNA與RNA的比例，即可得知rRNA/rDNA。本研究會先利用實驗室培養實驗來釐清 18S rRNA/rDNA與成長率之關係，之後應用在野外樣本。可讓我們對於超微真核藻類在實驗室與野外的生理狀態有更深入的解析。量測單一群的超微真核藻類之成長率可釐清在微生物循環中能量傳遞之動態。以上研究有助於了解副熱帶沿岸生態系中超微真核藻類在微生物食物網所扮演的功能性角色。此外將利用NGS技術去比較有光與無光條件下轉錄體差異，希望了解光合作用在混營營養行為中扮演之角色。 The photosynthetic picoeukaryotes (PPEs) provide high quantity of biomass and prominent degree of biodiversity for the world ocean. Also, they are the important primary producer in marine ecosystem. Study on the growth condition of PPEs allows us to understand their ecological distribution and carbon cycles. Ribosomal RNA (rRNA) has been regarded as a proxy for metabolic activity and population growth in the microbes, however, this technique is seldom applied to the PPEs. The 18S rRNA/rDNA ratio of PPEs will be measured against a known concentration of exogenous RNA/DNA standard. Subsequently, the quantities of 18S rRNA, rDNA, and exogenous standard will be determined via quantitative polymerase chain reaction (qPCR). First, the relationship between rRNA/rDNA and growth rate will be delineated through culture experiments in laboratory and then applied this result to the field samples. This measured ratio will provide a comprehensive view of the physiological status of PPEs in laboratory as well as in nature. The goal of this study on the growth rate at taxonomic levels is to measure and clarify the energy flux in the microbial loop. It will advance our understanding on the functional roles of PPEs community in the microbial food web of a subtropical neritic ecosystem. Furthermore, we shall apply the NGS (Next Generation Sequencing) to compare the difference of transcriptom under light and dark conditions to understand the role of photosynthesis in the mixotrophic behaviour.

Keyword(s)

成長
混營性
超微真核生物
rRNA/rDNA
growth
mixotrophy
photosynthetic picoeukaryotes
rRNA/rDNA

DSpace CRIS

Plankton Ecological Processes in Subtropical-Shelf Ecosystem: Metabolism Activity and Mixotrophic Mechanism of Picoeukaryotes(III)

基本資料

Description