http://scholars.ntou.edu.tw/handle/123456789/10009
標題: | Identification of six common species of processed filefish using cytochrome b gene sequence and PCR-RFLP analysis | 作者: | Ya-Jung Wu Cheng-Hong Hsieh Hong-Ming Chen Deng-Fwu Hwang |
關鍵字: | Filefish;Cytochrome b gene;PCR-RFLP;Species identifi cation | 公開日期: | 十二月-2008 | 出版社: | SAGE journals | 卷: | 19 | 起(迄)頁: | 151-158 | 來源出版物: | The Raffles Bulletin of Zoology | 摘要: | Sequence analysis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique were used to identify the commercial species of Aluterus monoceros, Aluterus scriptus, Monacanthus chinensis, Thamnaconus hypargyreus, Thamnaconus modestus and Chaetodermis penicilligerus. The primers L14735 and H15149ad were designed in the mitochondrial cytochrome b gene and the molecular weight of amplifi ed fragment from processed fi lefi sh meats was 465 bp. Furthermore, the results obtained from the HaeIII enzyme digestion could be used to distinguish the six commercial fi lefi sh species in fresh and heat-treated meats. Using the PCR-RFLP method, six fi lefi sh species could be successfully identifi ed in fresh and heat-treated meats. The PCR-RFLP technique developed in this study was proved to be a rapid, reliable and simple method that enables easy and accurate identifi cation of six commercial fi lefi sh processed species. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/10009 | ISSN: | 0217-2445 |
顯示於: | 食品科學系 |
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