http://scholars.ntou.edu.tw/handle/123456789/10267
標題: | Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells | 作者: | Cheng, Tsai-Mu Li, Rou Kao, Yu-Chieh Jill Hsu, Chun-Hua Chu, Hsueh-Liang Lu, Kun-Ying Changou, Chun A. Chang, Che-Chang Chang, Lee-Hsin Tsai, Min-Lang Mi, Fwu-Long |
關鍵字: | P-SELECTIN;ANTIOXIDANT ACTIVITY;CIRCULATION TIME;CONTRAST AGENT;FUCOIDAN;DELIVERY;CHITOSAN;EXPRESSION;CONJUGATE;THERAPIES | 公開日期: | 九月-2020 | 出版社: | ELSEVIER | 卷: | 114 | 來源出版物: | MAT SCI ENG C-MATER | 摘要: | P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed prolamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNP5). The formation of TPP1880/LMWF8775 CNP5 was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNP5 selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNP5 with better T-1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNP5 may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/10267 | ISSN: | 0928-4931 | DOI: | 10.1016/j.msec.2020.111064 |
顯示於: | 食品科學系 03 GOOD HEALTH AND WELL-BEING |
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