http://scholars.ntou.edu.tw/handle/123456789/12276
標題: | Spermatogonial transplantation in fish: A novel method for the preservation of genetic resources | 作者: | Yoshizaki, G. Fujinuma, K. Iwasaki, Y. Okutsu, T. Shinya Shikina Yazawa, R. Takeuchi, Y. |
公開日期: | 三月-2011 | 出版社: | Elsevier | 卷: | 6 | 期: | 1 | 起(迄)頁: | 55-61 | 來源出版物: | Comparative Biochemistry and Physiology D-Genomics & Proteomics | 摘要: | Recent progress in genome-based breeding has created various fish strains carrying desirable genetic traits; however, methods for the long-term preservation of their genetic resources have not yet been developed, mainly due to the lack of cryopreservation techniques for fish eggs and embryos. Recently, we established an alternative cryopreservation technique for fish spermatogonia using a slow-freezing method. Furthermore, we developed a transplantation system to produce functional eggs and sperm derived from spermatogonia. Spermatogonia isolated from the testes of vasa-green fluorescent protein (Gfp) transgenic rainbow trout (Oncorhynchus mykiss) were transplanted into the peritoneal cavity of triploid masu salmon (Oncorhynchus masou) hatchlings of both genders. The transplanted trout spermatogonia migrated towards the gonadal anlagen of the recipient salmon, into which they were subsequently incorporated. We confirmed that the donor-derived spermatogonia resumed gametogenesis, and produced sperm and eggs in male and female recipient salmon, respectively. Fertilization of the resultant eggs and sperm produced only rainbow trout in the first filial (F₁) generation, suggesting that the sterile triploid recipient salmon produced functional eggs and sperm derived from the trout donors. A combination of spermatogonial transplantation and cryopreservation could be a powerful tool for preserving valuable fish strains with desirable genetic traits and endangered species. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/12276 | ISSN: | 1744-117X | DOI: | 10.1016/j.cbd.2010.05.003 |
顯示於: | 海洋環境與生態研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。