|Title:||Application of honey in cryopreservation of sperm of milkfish (Chanos chanos) and black porgy (Acanthopagrus schlegeli). In：D.R. Idler, L.W. Crim and J.M. Walsh (eds.)||Authors:||Chao, N.H
|Issue Date:||1987||Publisher:||Proceedings of the Third International Symposium on the Reproductive Physiology of Fish||Abstract:||
For cryopreserving sperm of marine fin-fish, extenders are needed to dilute viscid milt and to provide nutrients for long-term storage. The aim is to find a more functional, convenient and inexpensive extender for milkfish and black porgy sperm preservation.
Pure honey is not only a natural bacteria inhibitor but also reasonably priced and readily available. The result of this study shows that 1% honey (449 mOsm/kg) was an optimum substitute for milkfish serum in the preserving of milkfish sperm in terms of prefreezing and post-thawing motility. Nevertheless, fertility needs to be studied in the near future. On the other hand, 0.5% honey (210 mOsm/kg) was used to substitute 5.6% glucose in preserving black porgy sperm and rather satisfactory prefreezing, frozen-thawed, and three-day frozen-thawed motility as well as fertility were obtained.
|Appears in Collections:||海洋中心|
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