http://scholars.ntou.edu.tw/handle/123456789/14409
標題: | Electroporation of sperm to introduce foreign DNA into the genome of loach (Misgurnus anguillicaudatus) | 作者: | Huai-Jen Tsai F. S. Tseng I-Chiu Liao |
公開日期: | 1995 | 出版社: | Canadian Journal of Fisheries and Aquatic Sciences | 引用: | Electroporation of sperm to introduce foreign DNA into the genome of loach... | Request PDF. Available from: https://www.researchgate.net/publication/237184612_Electroporation_of_sperm_to_introduce_foreign_DNA_into_the_genome_of_loach_Misgurnus_anguillicaudatus [accessed Dec 13 2017]. | 起(迄)頁: | 52(4), pp.776-787 | 摘要: | An antifreeze protein gene promoter from ocean pout (Macrozoarces americanus) linked to a chinook salmon (Oncorhynchus tshawytscha) growth hormone cDNA (opAFPGHc) was introduced into loach (Misgurnus anguillicaudatus) sperm via electroporation under the following conditions: amplitude, 8 kV; burst time, 1.6 s; number of pulses; 28; pulse time, 120 μs; cycle, 6. Compared with the untreated sperm, the fertilization rate driven by the electroporated sperm was decreased slightly, with no significant effect on hatchability. The success rate of gene transfer was 50%. Genomic Southern blotting demonstrated that DNA from transgenic loach contained positive band(s) with higher (37.5%), the same, or lower molecular size as the transferred opAFPGHc fragment. There was a mosaic distribution of exogenous DNA in a wide variety of tissues analyzed. The average weight gain between the 14th and 161th day of the experimental group was 2.5-fold higher than those of the control and mock-treated groups. The body length distribution of the experimental group was rather widely scattered instead of being normally distributed. |
URI: | ntour.ntou.edu.tw:8080/ir/handle/987654321/44905 http://scholars.ntou.edu.tw/handle/123456789/14409 |
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