http://scholars.ntou.edu.tw/handle/123456789/15244
Title: | Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection | Authors: | Kuan-Yu Chen Tai-Ching Hsu Po-Yu Huang Shih-Ting Kang Chu-Fang Lo Wei-Pang Huang Li-Li Chen |
Keywords: | white spot syndrome virus (wssv);envelope protein;vp53a;Penaeus monodon chitin-binding protein (PmCBP);In vivo neutralization | Issue Date: | Sep-2009 | Publisher: | Elsevier | Journal Volume: | 27 | Journal Issue: | 3 | Start page/Pages: | 460-465 | Source: | Fish & Shellfish Immunology | Abstract: | White spot syndrome virus (WSSV) can cause the most serious viral disease of shrimp and has a wide host range among crustaceans. Although researches show a lot about its genome and structure, information concerning the mechanism of how WSSV infects' cells is lacking. In this study, some experiments were applied to confirm the biological meaning of the protein–protein interaction between WSSV envelope protein, VP53A, and Penaeus monodon chitin-binding protein (PmCBP). Immunofluorescent study indicated that PmCBP is located on the cell surface of host cells. PmCBP amounts of about 34 kDa can be detected in both P. monodon and Litopenaeus vannamei tissues by Western blotting. In the in vivo neutralization experiment, both rVP53A and rPmCBP that were produced by Esherichia coli can promote resp. a 40% and 20% survival rate of the shrimp which were challenged by WSSV. Furthermore, a yeast-two-hybrid result revealed that PmCBP could interact with at least 11 WSSV envelope proteins. Those findings suggest that PmCBP may be involved in WSSV infection. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/15244 | DOI: | https://doi.org/10.1016/j.fsi.2009.06.018 |
Appears in Collections: | 海洋生物研究所 |
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