http://scholars.ntou.edu.tw/handle/123456789/16098
標題: | Functional characterization of myeloid differentiation factor 88 in Nile tilapia (Oreochromis niloticus) | 作者: | Nguyen Bao Trung Lee, Po-Tsang |
關鍵字: | TOLL-LIKE RECEPTORS;GENE-EXPRESSION;SUBCELLULAR-LOCALIZATION;MYD88;ADAPTERS;IFN;RECOGNITION;INNATE;LPS;TIR | 公開日期: | 十二月-2020 | 出版社: | ELSEVIER SCIENCE INC | 卷: | 250 | 來源出版物: | COMP BIOCHEM PHYS B | 摘要: | Myeloid differentiation factor 88 (MyD88) is an indispensable component of the toll-like receptor (TLR)- and interleukin 1 receptor-mediated signaling pathways and plays an important role in defensive immune responses. In this study, we cloned MyD88 from the Nile tilapia, Oreochromis niloticus (OnMyD88). Phylogenetic analysis showed that OnMyD88 is grouped with other teleost MyD88s and has the highest amino acid identity and similarity to fugu MyD88. The gene organization of OnMyD88 is similar to MyD88 in several other fish species, except for Atlantic salmon. Gene expression studies revealed that OnMyD88 is highly expressed in immunerelated tissues, such as spleen and head kidney, and is up-regulated in spleen and head kidney tissues from fish infected by Streptococcus agalactiae. Functional studies showed that overexpressed OnMyD88 could activate NF-kappa B and AP-1 in human embryonic kidney (HEK) 293 and tilapia head kidney (THK) cell lines and induce the expression of proinflammatory cytokines such as IL-1 beta and IL-12b and chemokine IL-8 in THK cells. The result of the co-immunoprecipitation demonstrated that OnMyD88 can be recruited by OnTLR25 as its adaptor. Taken together, our study indicates that OnMyD88 plays an essential role in the innate immune responses of Nile tilapia against bacterial infection. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/16098 | ISSN: | 1096-4959 | DOI: | 10.1016/j.cbpb.2020.110485 |
顯示於: | 水產養殖學系 14 LIFE BELOW WATER |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。