|Title:||Sinking of Four Species of Living Diatom Cells Directly Observed by a "Tumbled" Optical Microscope||Authors:||Hamano, Ryo
|Keywords:||diatom;microchamber;optical microscope;trajectory||Issue Date:||1-Oct-2021||Publisher:||CAMBRIDGE UNIV PRESS||Journal Volume:||27||Journal Issue:||5||Start page/Pages:||1154-1160||Source:||MICROSCOPY AND MICROANALYSIS||Abstract:||
The study of the sinking phenomenon of diatom cells, which have a slightly larger specific gravity (similar to 1.3) compared to that of water, is an important research topic for understanding photosynthetic efficiency. In this study, we successfully demonstrated the observation of the sinking behaviors of four different species of diatom using a homemade "tumbled" optical microscope. A homemade 1 mm(3) microchamber was employed to decrease the effects of convection currents. In the microchamber, diatom cells were basically settled in a linear manner without floating, although some of the cells were rotated during their sinking. Sinking speeds of the four species of diatom cells, Nitzschia sp., Pheodactylum tricornutum, Navicula sp., and Odontella aurita, were 0.81 +/- 5.56, 3.03 +/- 10.17, 3.29 +/- 7.39, and 11.22 +/- 21.42 mu m/s, respectively, based on the automatic tracking analysis of the centroids of each cell. Manual analysis of a vector between two longitudinal ends of the cells (two-point analysis) was effective for quantitatively characterizing the rotation phenomenon; therefore, angles and angular velocities of rotating cells were well determined as a function of time. The effects of the cell shapes on sinking velocity could be explained by simulation analysis using the modified Stokes' law proposed by Miklasz et al.
|Appears in Collections:||生命科學暨生物科技學系|
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